ArticleThe EMBO journal2004
Molecular mechanism of voltage sensor movements in a potassium channel.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Voltage vs. Ligand I: Structural basis of the intrinsic flexibility of S3 segment and its significance in ion channel activation.Channels (Austin, Tex.) · 2019Article
- Article
- Conformational changes during human P2X7 receptor activation examined by structural modelling and cysteine-based cross-linking studies.Purinergic signalling · 2017Article
- Energetic role of the paddle motif in voltage gating of Shaker K(+) channels.Nature structural & molecular biology · 2013Article
- Voltage sensor ring in a native structure of a membrane-embedded potassium channel.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Orientations and proximities of the extracellular ends of transmembrane helices S0 and S4 in open and closed BK potassium channels.PloS one · 2013Article
- Acute Ca(2+)-dependent desensitization of 5-HT(1A) receptors is mediated by activation of protein kinase A (PKA) in rat serotonergic neurons.Neuroscience · 2010Article
- Probing S4 and S5 segment proximity in mammalian hyperpolarization-activated HCN channels by disulfide bridging and Cd2+ coordination.Pflugers Archiv : European journal of physiology · 2009Article
- Position and role of the BK channel alpha subunit S0 helix inferred from disulfide crosslinking.The Journal of general physiology · 2008Article
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- Article
- Mapping the membrane-aqueous border for the voltage-sensing domain of a potassium channel.The Journal of biological chemistry · 2007Article
- Acidic residues on the voltage-sensor domain determine the activation of the NaChBac sodium channel.Biophysical journal · 2007Article
- Accessibility of four arginine residues on the S4 segment of the Bacillus halodurans sodium channel.The Journal of membrane biology · 2007Article
- Molecular template for a voltage sensor in a novel K+ channel. I. Identification and functional characterization of KvLm, a voltage-gated K+ channel from Listeria monocytogenes.The Journal of general physiology · 2006Article
- Voltage sensor conformations in the open and closed states in ROSETTA structural models of K(+) channels.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- S3b amino acid residues do not shuttle across the bilayer in voltage-dependent Shaker K+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-gated potassium channels are six-transmembrane (S1-S6) proteins that form a central pore domain (4 x S5-S6) surrounded by four voltage sensor domains (S1-S4), which detect changes in membrane voltage and control pore opening. Upon depolarization, the S4 segments move outward carrying charged residues across the membrane field, thereby leading to the opening of the pore. The mechanism of S4 motion is controversial. We have investigated how S4 moves relative to the pore domain in the prototypical Shaker potassium channel. We introduced pairs of cysteines, one in S4 and the other in S5, and examined proximity changes between each pair of cysteines during activation, using Cd2+ and copper-phenanthroline, which crosslink the cysteines with metal and disulphide bridges, respectively. Modelling of the results suggests a novel mechanism: in the resting state, the top of the S3b-S4 voltage sensor paddle lies close to the top of S5 of the adjacent subunit, but moves towards the top of S5 of its own subunit during depolarization--this motion is accompanied by a reorientation of S4 charges to the extracellular phase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.