ArticleJournal of virology2004
C-terminal nsP1a protein of human astrovirus colocalizes with the endoplasmic reticulum and viral RNA.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Three-dimensional peptide modeling and subcellular localization of the VPg protein of human astrovirus 8 in infected Caco-2 cells.Molecular biology reports · 2026Article
- The dynamics and strategy of RNA replication in astroviruses.NAR molecular medicine · 2026Article
- Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation.PLoS pathogens · 2025Article
- Isolation and identification of a new porcine astrovirus 5 demonstrated that oxidative stress enhances porcine astrovirus replication.BMC veterinary research · 2025Article
- Identification of viral protease-dependent cleavage sites within the human astrovirus polyprotein.Journal of virology · 2025Article
- NEDD4 family ubiquitin ligase AIP4 interacts with Alix to enable HBV naked capsid egress in an Alix ubiquitination-independent manner.PLoS pathogens · 2024Article
- Mouse and human immune responses share neutralization epitopes of HAstV-VA1.Journal of virology · 2024Article
- The astrovirus N-terminal nonstructural protein anchors replication complexes to the perinuclear ER membranes.PLoS pathogens · 2024Article
- Astrovirus replication is dependent on induction of double-membrane vesicles through a PI3K-dependent, LC3-independent pathway.Journal of virology · 2023Article
- Dual-fluorescent reporter for live-cell imaging of the ER during DENV infection.Frontiers in cellular and infection microbiology · 2022Article
- Targeting the Viral Polymerase of Diarrhea-Causing Viruses as a Strategy to Develop a Single Broad-Spectrum Antiviral Therapy.Viruses · 2019Review
- Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis.Scientific reports · 2018Article
- The Ubiquitin-Proteasome System Is Necessary for Efficient Replication of Human Astrovirus.Journal of virology · 2018Article
- Studies on the Virome of the Entomopathogenic Fungus Beauveria bassiana Reveal Novel dsRNA Elements and Mild Hypervirulence.PLoS pathogens · 2017Article
- The Immune Response to Astrovirus Infection.Viruses · 2016Review
- Type I Interferon Response Limits Astrovirus Replication and Protects against Increased Barrier Permeability In Vitro and In Vivo.Journal of virology · 2016Article
- Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells.Journal of virology · 2015Article
- New insights on the role of paired membrane structures in coronavirus replication.Virus research · 2015Article
- Article
- Does form meet function in the coronavirus replicative organelle?Trends in microbiology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Computational and biological approaches were undertaken to characterize the role of the human astrovirus nonstructural protein nsP1a/4, located at the C-terminal fragment of nsP1a. Computer analysis reveals sequence similarities to other nonstructural viral proteins involved in RNA replication and/or transcription and allows the identification of a glutamine- and proline-rich region, the prediction of many phosphorylation and O-glycosylation sites, and the occurrence of a KKXX-like endoplasmic reticulum retention signal. Immunoprecipitation analysis with an antibody against a synthetic peptide of the nsP1a/4 sequence detected polyprotein precursors of 160, 75, and 38 to 40 kDa as well as five smaller proteins in the range of 21 to 27 kDa. Immunofluorescence labeling showed that the nsP1a/4 protein is accumulated at the perinuclear region, in association with the endoplasmic reticulum and the viral RNA. These results suggest the involvement of nsP1a/4 protein in the RNA replication process in endoplasmic reticulum-derived intracellular membranes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.