ArticlePlant physiology2004
Inactivation of the clpC1 gene encoding a chloroplast Hsp100 molecular chaperone causes growth retardation, leaf chlorosis, lower photosynthetic activity, and a specific reduction in photosystem content.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 145 citations in OpenAlex.
- Correlated Evolutionary Rates Reveal Novel Components and Cross-Compartment Connectivity in Plant Proteostasis Systems.Genome biology and evolution · 2026Article
- Uvr motifs regulate the chloroplast Clp chaperone-protease system.Trends in plant science · 2025Review
- A high-density linkage map and fine QTL mapping of architecture, phenology, and yield-related traits in faba bean (Frontiers in plant science · 2025Article
- Genome-wide identification and expression analysis of heat shock protein gene family in cassava.The plant genome · 2023Article
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.Plant communications · 2023Review
- Chloroplasts Protein Quality Control and Turnover: A Multitude of Mechanisms.International journal of molecular sciences · 2022Review
- Comparative analysis of differential gene expression indicates divergence in ontogenetic strategies of leaves in two conifer genera.Ecology and evolution · 2022Article
- Changes in the Flower and Leaf Proteome of Common Buckwheat (International journal of molecular sciences · 2021Article
- OsNBL1, a Multi-Organelle Localized Protein, Plays Essential Roles in Rice Senescence, Disease Resistance, and Salt Tolerance.Rice (New York, N.Y.) · 2021Article
- Article
- A Long-Day Photoperiod and 6-Benzyladenine Promote Runner Formation through Upregulation of Soluble Sugar Content in Strawberry.International journal of molecular sciences · 2020Article
- Protective Roles of Cytosolic and Plastidal Proteasomes on Abiotic Stress and Pathogen Invasion.Plants (Basel, Switzerland) · 2020Review
- Protein Import Motors in Chloroplasts: On the Role of Chaperones.The Plant cell · 2020Article
- Co-Suppression ofPlants (Basel, Switzerland) · 2020Article
- The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response.eLife · 2019Article
- Co-suppression of NbClpC1 and NbClpC2 alters plant morphology with changed hormone levels in Nicotiana benthamiana.Plant cell reports · 2019Article
- En route into chloroplasts: preproteins' way home.Photosynthesis research · 2018Review
- Farnesylated heat shock protein 40 is a component of membrane-bound RISC inThe Journal of biological chemistry · 2018Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ClpC is a molecular chaperone of the Hsp100 family. In higher plants there are two chloroplast-localized paralogs (ClpC1 and ClpC2) that are approximately 93% similar in primary sequence. In this study, we have characterized two independent Arabidopsis (Arabidopsis thaliana) clpC1 T-DNA insertion mutants lacking on average 65% of total ClpC content. Both mutants display a retarded-growth phenotype, leaves with a homogenous chlorotic appearance throughout all developmental stages, and more perpendicular secondary influorescences. Photosynthetic performance was also impaired in both knockout lines, with relatively fewer photosystem I and photosystem II complexes, but no changes in ATPase and Rubisco content. However, despite the specific drop in photosystem I and photosystem II content, no changes in leaf cell anatomy or chloroplast ultrastructure were observed in the mutants compared to the wild type. Previously proposed functions for envelope-associated ClpC in chloroplast protein import and degradation of mistargeted precursors were examined and shown not to be significantly impaired in the clpC1 mutants. In the stroma, where the majority of ClpC protein is localized, marked increases of all ClpP paralogs were observed in the clpC1 mutants but less variation for the ClpR paralogs and a corresponding decrease in the other chloroplast-localized Hsp100 protein, ClpD. Increased amounts of other stromal molecular chaperones (Cpn60, Hsp70, and Hsp90) and several RNA-binding proteins were also observed. Our data suggest that overall ClpC as a stromal molecular chaperone plays a vital role in chloroplast function and leaf development and is likely involved in photosystem biogenesis.
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