ArticleMolecular biology of the cell2005
Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 58 citations in OpenAlex.
- The annulus: composition, role and importance in sperm flagellum biogenesis and male fertility.Basic and clinical andrology · 2024Review
- Loss of an extensive ciliary connectome induces proteostasis and cell fate switching in a severe motile ciliopathy.bioRxiv : the preprint server for biology · 2024Article
- Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia.Nature communications · 2024Article
- J-like protein family of Arabidopsis thaliana: the enigmatic cousins of J-domain proteins.Plant cell reports · 2022Review
- Structures of radial spokes and associated complexes important for ciliary motility.Nature structural & molecular biology · 2021Article
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- Comparative genomic analysis of the 'pseudofungus'Open biology · 2018Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
- Mutations in DNAJB13, Encoding an HSP40 Family Member, Cause Primary Ciliary Dyskinesia and Male Infertility.American journal of human genetics · 2016Article
- A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures.PLoS genetics · 2015Article
- In situ localization of N and C termini of subunits of the flagellar nexin-dynein regulatory complex (N-DRC) using SNAP tag and cryo-electron tomography.The Journal of biological chemistry · 2015Article
- Assessment of the frequency of sperm annulus defects in a large cohort of patients presenting asthenozoospermia.Basic and clinical andrology · 2015Article
- The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking.Cytoskeleton (Hoboken, N.J.) · 2014Article
- DNAJB13, a type II HSP40 family member, localizes to the spermatids and spermatozoa during mouse spermatogenesis.BMC developmental biology · 2014Article
- The Chlamydomonas mutant pf27 reveals novel features of ciliary radial spoke assembly.Cytoskeleton (Hoboken, N.J.) · 2013Article
- The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans.Nature genetics · 2013Article
- Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Axonemal radial spokes: 3D structure, function and assembly.Bioarchitecture · 2012Article
- Article
- Cryoelectron tomography of radial spokes in cilia and flagella.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The radial spoke is a stable structural complex in the 9 + 2 axoneme for the control of flagellar motility. However, the spokes in Chlamydomonas mutant pf24 are heterogeneous and unstable, whereas several spoke proteins are reduced differentially. To elucidate the defective mechanism, we clone RSP16, a prominent spoke protein diminished in pf24 axonemes. Unexpectedly, RSP16 is a novel HSP40 member of the DnaJ superfamily that assists chaperones in various protein-folding-related processes. Importantly, RSP16 is uniquely excluded from the 12S spoke precursor complex that is packaged in the cell body and transported toward the flagellar tip to be converted into mature 20S axonemal spokes. Rather, RSP16, transported separately, joins the precursor complex in flagella. Furthermore, RSP16 molecules in vitro and in flagella form homodimers, a characteristic required for the cochaperone activity of HSP40. We postulate that the spoke HSP40 operates as a cochaperone to assist chaperone machinery at the flagellar tip to actively convert the smaller spoke precursor and itself into the mature stable complex; failure of the interaction between the spoke HSP40 and its target polypeptide results in heterogeneous unstable radial spokes in pf24.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.