ArticleMolecular biology of the cell2005
The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 97 citations in OpenAlex.
- Transcriptomics analysis identifies folding and secretion related genes for improving monoclonal antibody production in Thermothelomyces heterothallica C1.Microbial cell factories · 2026Article
- TOR complex 1 negatively regulates NDR kinase Cbk1 to control cell separation in budding yeast.PLoS biology · 2023Article
- Phosphorylation of Pal2 by the protein kinases Kin1 and Kin2 modulatesScience signaling · 2021Article
- A Snf1-related nutrient-responsive kinase antagonizes endocytosis in yeast.PLoS genetics · 2020Article
- Phospholipids of the Plasma Membrane - Regulators or Consequence of Cell Polarity?Frontiers in cell and developmental biology · 2020Review
- Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase.Cells · 2019Article
- Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.Cell reports · 2019Article
- Adaptation to Endoplasmic Reticulum Stress Requires Transphosphorylation within the Activation Loop of Protein Kinases Kin1 and Kin2, Orthologs of Human Microtubule Affinity-Regulating Kinase.Molecular and cellular biology · 2018Article
- Substrate priming enhances phosphorylation by the budding yeast kinases Kin1 and Kin2.The Journal of biological chemistry · 2018Article
- Identification of stress responsive genes by studying specific relationships between mRNA and protein abundance.Heliyon · 2018Article
- Phosphoinositides and Membrane Targeting in Cell Polarity.Cold Spring Harbor perspectives in biology · 2018Review
- Identifying protein kinase-specific effectors of the osmostress response in yeast.Science signaling · 2017Article
- Molecular determinants of KA1 domain-mediated autoinhibition and phospholipid activation of MARK1 kinase.The Biochemical journal · 2017Article
- Rab11-FIP1 phosphorylation by MARK2 regulates polarity in MDCK cells.Cellular logistics · 2017Article
- Regulation of Cell Polarity by PAR-1/MARK Kinase.Current topics in developmental biology · 2017Review
- The eukaryotic protein kinase superfamily of the necrotrophic fungal plant pathogen, Sclerotinia sclerotiorum.Molecular plant pathology · 2016Review
- The Kinome of Edible and Medicinal FungusFrontiers in microbiology · 2016Article
- Article
- Structural insight into the mechanism of synergistic autoinhibition of SAD kinases.Nature communications · 2015Article
- A novel role for protein kinase Kin2 in regulating HAC1 mRNA translocation, splicing, and translation.Molecular and cellular biology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Kin1 and Kin2 are Saccharomyces cerevisiae counterparts of Par-1, the Caenorhabditis elegans kinase essential for the establishment of polarity in the one cell embryo. Here, we present evidence for a novel link between Kin1, Kin2, and the secretory machinery of the budding yeast. We isolated KIN1 and KIN2 as suppressors of a mutant form of Rho3, a Rho-GTPase acting in polarized trafficking. Genetic analysis suggests that KIN1 and KIN2 act downstream of the Rab-GTPase Sec4, its exchange factor Sec2, and several components of the vesicle tethering complex, the Exocyst. We show that Kin1 and Kin2 physically interact with the t-SNARE Sec9 and the Lgl homologue Sro7, proteins acting at the final stage of exocytosis. Structural analysis of Kin2 reveals that its catalytic activity is essential for its function in the secretory pathway and implicates the conserved 42-amino acid tail at the carboxy terminal of the kinase in autoinhibition. Finally, we find that Kin1 and Kin2 induce phosphorylation of t-SNARE Sec9 in vivo and stimulate its release from the plasma membrane. In summary, we report the finding that yeast Par-1 counterparts are associated with and regulate the function of the exocytic apparatus via phosphorylation of Sec9.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.