ArticleMolecular biology of the cell2005
Molecular characterization of radial spoke subcomplex containing radial spoke protein 3 and heat shock protein 40 in sperm flagella of the ascidian Ciona intestinalis.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 62 citations in OpenAlex.
- Heterogeneity of radial spoke components in Tetrahymena cilia.Cellular and molecular life sciences : CMLS · 2025Article
- Molecular chaperone Hsp90 protects KCBP from degradation by proteasome in Dunaliella salina cells.Folia microbiologica · 2021Article
- Distinct architecture and composition of mouse axonemal radial spoke head revealed by cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.Cells · 2019Review
- ARC3 Activation by PARC6 Promotes FtsZ-Ring Remodeling at the Chloroplast Division Site.The Plant cell · 2019Article
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Article
- The structural heterogeneity of radial spokes in cilia and flagella is conserved.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Cryoelectron tomography of radial spokes in cilia and flagella.The Journal of cell biology · 2011Article
- A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis.PloS one · 2010Article
- CCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena.PloS one · 2010Article
- Chlamydomonas mutants display reversible deficiencies in flagellar beating and axonemal assembly.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Global gene expression profiles for life stages of the deadly amphibian pathogen Batrachochytrium dendrobatidis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella.The Journal of cell biology · 2008Article
- Radial spoke protein 44 (human meichroacidin) is an axonemal alloantigen of sperm and cilia.Gene · 2007Article
- Radial spoke proteins of Chlamydomonas flagella.Journal of cell science · 2006Article
- A genomewide analysis of genes for the heat shock protein 70 chaperone system in the ascidian Ciona intestinalis.Cell stress & chaperones · 2006Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the heat-shock protein (HSP)40 regulate the protein folding activity of HSP70 proteins and help the functional specialization of this molecular chaperone system in various types of cellular events. We have recently identified Hsp40 as a component of flagellar axoneme in the ascidian Ciona intestinalis, suggesting a correlation between Hsp40 related chaperone system and flagellar function. In this study, we have found that Ciona 37-kDa Hsp40 is extracted from KCl-treated axonemes with 0.5 M KI solution and comigrates with radial spoke protein (RSP)3 along with several proteins as a complex through gel filtration and ion exchange columns. Peptide mass fingerprinting with matrix-assisted laser desorption ionization/time of flight/mass spectrometry revealed that other proteins in the complex include a homolog of sea urchin spokehead protein (homolog of RSP4/6), a membrane occupation and recognition nexus repeat protein with sequence similarity with meichroacidin, and a functionally unknown 33-kDa protein. A spoke head protein, LRR37, is not included in the complex, suggesting that the complex constructs the stalk of radial spoke. Immunoelectron microscopy indicates that Hsp40 is localized in the distal portion of spoke stalk, possibly at the junction between spoke head and the stalk.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.