ArticleNucleic acids research2004
Bigenic Cre/loxP, puDeltatk conditional genetic ablation.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Type II collagen-positive progenitors are important stem cells in controlling skeletal development and vascular formation.Bone research · 2022Article
- CNS fibroblasts form a fibrotic scar in response to immune cell infiltration.Nature neuroscience · 2021Article
- Connecting teratogen-induced congenital heart defects to neural crest cells and their effect on cardiac function.Birth defects research. Part C, Embryo today : reviews · 2014Review
- Molecular neuroanatomy's "Three Gs": a primer.Neuron · 2007Review
- Use of transgenic animals to improve human health and animal production.Reproduction in domestic animals = Zuchthygiene · 2005Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic ablation experiments are used to resolve problems regarding cell lineages and the in vivo function of certain groups of cells. We describe a two-component conditional ablation technology using a mouse carrying an X-linked puDeltatk transgene, which is only activated in cells expressing Cre. Ablation of the Cre-expressing cells can be temporally regulated by the time of ganciclovir (GCV) administration. This strategy was demonstrated using a Col2Cre transgenic line. Differentiating chondrocytes in bigenic animals could be ablated at different developmental stages resulting in disorganized growth plates and dwarfism. Macrocephaly, macroglossia and umbilical hernia were also observed in ablated 18.5 dpc embryos. Crosses between the puDeltatk selector transgenic line and existing cre lines will facilitate numerous temporally regulated tissue-specific ablation experiments.
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