ArticleThe Journal of clinical investigation1992
Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes.
Article in The Journal of clinical investigation, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 139 citations in OpenAlex.
- The Hydropathy Index of the HCDR3 Region of the B-Cell Receptor Identifies Two Subgroups of IGHV-Mutated Chronic Lymphocytic Leukemia Patients With Distinct Outcome.Frontiers in oncology · 2021Article
- Structure, Function, and Therapeutic Use of IgM Antibodies.Antibodies (Basel, Switzerland) · 2020Review
- Memory B cell heterogeneity: Remembrance of things past.Journal of leukocyte biology · 2018Review
- 5' Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice.Frontiers in immunology · 2018Article
- Translating transitions - how to decipher peripheral human B cell development.Journal of biomedical research · 2015Review
- Protein A-specific monoclonal antibodies and prevention of Staphylococcus aureus disease in mice.Infection and immunity · 2012Article
- Epstein-Barr virus infection of naïve B cells in vitro frequently selects clones with mutated immunoglobulin genotypes: implications for virus biology.PLoS pathogens · 2012Article
- Potential of a unique antibody gene signature to predict conversion to clinically definite multiple sclerosis.Journal of neuroimmunology · 2009Article
- CSF IgG heavy-chain bias in patients at the time of a clinically isolated syndrome.Journal of neuroimmunology · 2008Article
- Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.Journal of immunological methods · 2008Article
- Analysis of the expressed heavy chain variable-region genes of Macaca fascicularis and isolation of monoclonal antibodies specific for the Ebola virus' soluble glycoprotein.Immunogenetics · 2005Article
- IGHV1, IGHV5 and IGHV7 subgroup genes in the rhesus macaque.Immunogenetics · 2003Article
- Long-term kinetics of adult human antibody repertoires.Immunology · 2002Article
- Immunoglobulin heavy chain expression shapes the B cell receptor repertoire in human B cell development.The Journal of clinical investigation · 2001Article
- Impact of age on hypermutation of immunoglobulin variable genes in humans.Journal of clinical immunology · 2001Article
- Ig heavy chain CDR3 size diversities are similar after conventional peripheral blood and ex vivo expanded hematopoietic cell transplants.Bone marrow transplantation · 2001Article
- B lymphocyte involvement in ankylosing spondylitis: the heavy chain variable segment gene repertoire of B lymphocytes from germinal center-like foci in the synovial membrane indicates antigen selection.Arthritis research · 2001Article
- Article
- Human immunoglobulin (Ig)M+IgD+ peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells.The Journal of experimental medicine · 1998Article
- VH usage and somatic hypermutation in peripheral blood B cells of patients with rheumatoid arthritis (RA).Clinical and experimental immunology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
cDNA libraries for IgM heavy chain variable regions were prepared from unmanipulated peripheral blood lymphocytes of two healthy people. Partial sequencing of 103 clones revealed VH gene family use and complete CDR3 and JH sequences. The libraries differed in the two subjects. In one person's cDNA the VH5 family was overexpressed and the VH3 family underexpressed relative to genomic complexity. In the second person's cDNA, VH3 was most frequently expressed. In both libraries, JH4 was most frequent. VH segments of several clones were closely related to those in fetal repertoires. However, there was also evidence of mutation in many cDNAs. Three clones differed from the single nonpolymorphic VH6 germline gene by 7-13 bases. Clones with several differences from VH5 germline gene VH251 were identified. CDR3 segments were highly diverse. JH portions of several CDR3's differed from germline JH sequences. 44% of the clones had DH genes related to the DLR and DXP families, most with differences from germline sequences. In 11 DLR2-related sequences, several base substitutions could not be accounted for by polymorphism. Thus, circulating IgM-producing B cell populations include selected clones, some of which are encoded by variable region gene segments that have mutated from the germline form.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.