ArticleThe Journal of cell biology1992
Differential behavior of photoactivated microtubules in growing axons of mouse and frog neurons.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 105 citations in OpenAlex.
- Development and Application of Technology for Neural Circuit Visualization - Secondary Publication.JMA journal · 2024Review
- Moonlighting Motors: Kinesin, Dynein, and Cell Polarity.Trends in cell biology · 2017Review
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- A physical model of axonal elongation: force, viscosity, and adhesions govern the mode of outgrowth.Biophysical journal · 2008Article
- Direct evidence for coherent low velocity axonal transport of mitochondria.The Journal of cell biology · 2006Article
- Rapid redistribution of the postsynaptic density protein PSD-Zip45 (Homer 1c) and its differential regulation by NMDA receptors and calcium channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Glutamate slows axonal transport of neurofilaments in transfected neurons.The Journal of cell biology · 2000Article
- Reorganization and movement of microtubules in axonal growth cones and developing interstitial branches.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Different contributions of microtubule dynamics and transport to the growth of axons and collateral sprouts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Slow axonal transport of neurofilament protein in cultured neurons.The Journal of cell biology · 1999Article
- Dynamics of axonal microtubules regulate the topology of new membrane insertion into the growing neurites.The Journal of cell biology · 1998Article
- STOP proteins are responsible for the high degree of microtubule stabilization observed in neuronal cells.The Journal of cell biology · 1998Article
- Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Microtubule transport from the cell body into the axons of growing neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Delivery of newly synthesized tubulin to rapidly growing distal axons of sympathetic neurons in compartmented cultures.The Journal of cell biology · 1996Article
- Tubulin transport in neurons.The Journal of cell biology · 1996Article
- Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis.The Journal of cell biology · 1996Article
- Microtubule stability decreases axon elongation but not axoplasm production.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To characterize the behavior of axonal microtubules in vivo, we analyzed the movement of tubulin labeled with caged fluorescein after activation to be fluorescent by irradiation of 365-nm light. When mouse sensory neurons were microinjected with caged fluorescein-labeled tubulin and then a narrow region of the axon was illuminated with a 365-nm microbeam, photoactivated tubulin was stationary regardless of the position of photoactivation. We next introduced caged fluorescein-labeled tubulin into Xenopus embryos and nerve cells isolated from injected embryos were analyzed by photoactivation. In this case, movement of the photoactivated zone toward the axon tip was frequently observed. The photoactivated microtubule segments in the Xenopus axon moved out from their initial position without significant spreading, suggesting that fluorescent microtubules are not sliding as individual filaments, but rather translocating en bloc. Since these observations raised the possibility that the mechanism of nerve growth might differ between two types of neurons, we further characterized the movement of another component of the axon structure, the plasma membrane. Analysis of the position of polystyrene beads adhering to the neurites of Xenopus neurons revealed anterograde movement of the beads at the rate similar to the rate of microtubule movement. In contrast, no movement of the beads relative to the cell body was observed in mouse sensory neurons. These results suggest that the mode of translocation of cytoskeletal polymers and some components of the axon surface differ between two neuron types and that most microtubules are stationary within the axon of mammalian neurons where the surface-related motility of the axon is not observed.
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