ArticleHistochemistry and cell biology2004
Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland.
Article in Histochemistry and cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 55 citations in OpenAlex.
- Defining the electrical synapse.Nature reviews. Neuroscience · 2026Review
- High humidity environment increases FBG by impairing the intestinal barrier.Frontiers in immunology · 2025Article
- Article
- The Roles of Calmodulin and CaMKII in Cx36 Plasticity.International journal of molecular sciences · 2021Review
- Endocytosis of Connexin 36 is Mediated by Interaction with Caveolin-1.International journal of molecular sciences · 2020Article
- ZO-1 associates with α3 integrin and connexin43 in trabecular meshwork and Schlemm's canal cells.International journal of physiology, pathophysiology and pharmacology · 2020Article
- No tight junctions in tight junction protein-1 expressing HeLa and fibroblast cells.International journal of physiology, pathophysiology and pharmacology · 2020Article
- Gap junction protein connexin43 and tunneling nanotubes in human trabecular meshwork cells.International journal of physiology, pathophysiology and pharmacology · 2019Article
- Cancer Connectors: Connexins, Gap Junctions, and Communication.Frontiers in oncology · 2018Review
- The electrical synapse: Molecular complexities at the gap and beyond.Developmental neurobiology · 2017Review
- Roles of connexins and pannexins in (neuro)endocrine physiology.Cellular and molecular life sciences : CMLS · 2015Review
- Electrical synaptic transmission in developing zebrafish: properties and molecular composition of gap junctions at a central auditory synapse.Journal of neurophysiology · 2014Article
- Electrical synapses and their functional interactions with chemical synapses.Nature reviews. Neuroscience · 2014Review
- AII amacrine cells discriminate between heterocellular and homocellular locations when assembling connexin36-containing gap junctions.Journal of cell science · 2014Article
- Functional chromaffin cell plasticity in response to stress: focus on nicotinic, gap junction, and voltage-gated Ca2+ channels.Journal of molecular neuroscience : MN · 2012Review
- Under construction: building the macromolecular superstructure and signaling components of an electrical synapse.The Journal of membrane biology · 2012Article
- In vivo evidence for the involvement of the carboxy terminal domain in assembling connexin 36 at the electrical synapse.Molecular and cellular neurosciences · 2010Article
- Variability of distribution of Ca(2+)/calmodulin-dependent kinase II at mixed synapses on the mauthner cell: colocalization and association with connexin 35.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- The dual role of zonula occludens (ZO) proteins.Journal of biomedicine & biotechnology · 2010Review
- Revisiting the stimulus-secretion coupling in the adrenal medulla: role of gap junction-mediated intercellular communication.Molecular neurobiology · 2009Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PDZ domain-containing protein zonula occludens-1 (ZO-1), a well-established component of tight junctions, has recently been shown to interact with various connexin proteins that form gap junctions. We investigated the association of connexin36 (Cx36) with ZO-1 in various cultured cells and tissues. Punctate immunofluorescence labeling for Cx36 was detected in Cx36-transfected HeLa cells, betaTC-3 cells, pancreatic islets, and adrenal medulla. Immunofluorescence for ZO-1 was also punctate in cells and tissues, and was colocalized with Cx36 at points of cell-cell contact. Immunoprecipitation of either Cx36 or ZO-1 from cell lysates and tissue homogenates resulted in immunoblot detection of ZO-1 or Cx36, respectively, in immunoprecipitates. A 14-amino acid peptide corresponding to the carboxy-terminus of Cx36 showed binding capacity to the PDZ1 domain of ZO-1, which was eliminated after removal of the last 4 carboxy-terminus amino acids. Low micromolar concentrations of the 14-amino acid peptide produced up to 85% inhibition of Cx36 interaction with the PDZ1 domain of ZO-1. These results provide evidence for molecular interaction between Cx36 and ZO-1 in vitro, and in vivo, and suggest that the interference with Cx36/ZO-1 interaction by short carboxy-terminus peptides of Cx36 may be of value for functional studies of this interaction.
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