ArticleThe Journal of biological chemistry2005
Src phosphorylation of Alix/AIP1 modulates its interaction with binding partners and antagonizes its activities.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
- Adaptor protein CIN85 potentiates the motility of osteosarcoma cells via the Akt/mTOR and MMP2-COL3A1 axis.Molecular oncology · 2026Article
- Molecular Profiling of Extracellular Vesicles Isolated from Boar Reproductive Fluids.Journal of extracellular vesicles · 2026Article
- Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation.Science advances · 2023Article
- Insights into the function of ESCRT and its role in enveloped virus infection.Frontiers in microbiology · 2023Review
- Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4.Traffic (Copenhagen, Denmark) · 2022Article
- Review
- Budding of a Retrovirus: Some Assemblies Required.Viruses · 2020Review
- Proline-rich domain of human ALIX contains multiple TSG101-UEV interaction sites and forms phosphorylation-mediated reversible amyloids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- HCRP-1 regulates cell migration, invasion and angiogenesis via Src/ FAK signaling in human prostate cancer.International journal of biological sciences · 2020Article
- Src in endosomal membranes promotes exosome secretion and tumor progression.Scientific reports · 2019Article
- Syntenin mediates SRC function in exosomal cell-to-cell communication.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Phosphorylation-Dependent Activation of the ESCRT Function of ALIX in Cytokinetic Abscission and Retroviral Budding.Developmental cell · 2016Article
- Unravelling the pivotal role of Alix in MVB sorting and silencing of the activated EGFR.The Biochemical journal · 2015Article
- Nexus of signaling and endocytosis in oncogenesis driven by non-small cell lung cancer-associated epidermal growth factor receptor mutants.World journal of clinical oncology · 2014Review
- Membrane fission reactions of the mammalian ESCRT pathway.Annual review of biochemistry · 2013Review
- Analysis of SRC oncogenic signaling in colorectal cancer by stable isotope labeling with heavy amino acids in mouse xenografts.Molecular & cellular proteomics : MCP · 2012Article
- Structural recognition mechanisms between human Src homology domain 3 (SH3) and ALG-2-interacting protein X (Alix).FEBS letters · 2012Article
- A pathogen's journey in the host cell: Bridges between actin and traffic.Bioarchitecture · 2012Article
- EhADH112 is a Bro1 domain-containing protein involved in the Entamoeba histolytica multivesicular bodies pathway.Journal of biomedicine & biotechnology · 2012Article
- Proline-rich regions and motifs in trafficking: from ESCRT interaction to viral exploitation.Traffic (Copenhagen, Denmark) · 2011Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Alix/AIP1 is an adaptor protein involved in regulating the function of receptor and cytoskeleton-associated tyrosine kinases. Here, we investigated its interaction with and regulation by Src. Tyr319 of Alix bound the isolated Src homology-2 (SH2) domain and was necessary for interaction with intact Src. A proline-rich region in the C terminus of Alix bound the Src SH3 domain, but this interaction was dependent on the release of the Src SH2 domain from its Src internal ligand either by interaction with Alix Tyr319 or by mutation of Src Tyr527. Src phosphorylated Alix at a C-terminal region rich in tyrosines, an activity that was stimulated by the presence of the Alix binding partner SETA/CIN85. Phosphorylation of Alix by Src caused it to translocate from the membrane and cytoskeleton to the cytoplasm and reduced its interaction with binding partners SETA/CIN85, epidermal growth factor receptor, and Pyk2. As a consequence of this, Src antagonized the negative regulation of receptor tyrosine kinase internalization and cell adhesion by Alix. We propose a model whereby Src antagonizes the effects of Alix by phosphorylation of its C terminus, leading to the disruption of interactions with target proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.