ArticleThe Journal of biological chemistry2005
Evidence that monoclonal antibodies directed against the integrin beta subunit plexin/semaphorin/integrin domain stimulate function by inducing receptor extension.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions.Nature reviews. Molecular cell biology · 2023Review
- Regulation of integrin α5β1 conformational states and intrinsic affinities by metal ions and the ADMIDAS.Molecular biology of the cell · 2022Article
- Low-affinity integrin states have faster ligand-binding kinetics than the high-affinity state.eLife · 2021Article
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- Low-affinity binding inMolecular biology of the cell · 2017Article
- Ligand-induced Epitope Masking: DISSOCIATION OF INTEGRIN α5β1-FIBRONECTIN COMPLEXES ONLY BY MONOCLONAL ANTIBODIES WITH AN ALLOSTERIC MODE OF ACTION.The Journal of biological chemistry · 2016Article
- Relating conformation to function in integrin α5β1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Activation states of blood eosinophils in asthma.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2014Review
- Anti-IL-5 attenuates activation and surface density of β(2) -integrins on circulating eosinophils after segmental antigen challenge.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2013Article
- Integrin activation States and eosinophil recruitment in asthma.Frontiers in pharmacology · 2013Article
- Plexin structures are coming: opportunities for multilevel investigations of semaphorin guidance receptors, their cell signaling mechanisms, and functions.Cellular and molecular life sciences : CMLS · 2012Review
- Platelet activation, P-selectin, and eosinophil β1-integrin activation in asthma.American journal of respiratory and critical care medicine · 2012Article
- Overview: assays for studying integrin-dependent cell adhesion.Methods in molecular biology (Clifton, N.J.) · 2012Review
- Activation of beta1 integrins on blood eosinophils by P-selectin.American journal of respiratory cell and molecular biology · 2011Article
- Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.Journal of cell science · 2011Article
- Modulation of integrin activation by an entropic spring in the {beta}-knee.The Journal of biological chemistry · 2010Article
- Focal adhesions are sites of integrin extension.The Journal of cell biology · 2010Article
- Helicobacter pylori type IV secretion apparatus exploits beta1 integrin in a novel RGD-independent manner.PLoS pathogens · 2009Article
- Mapping the ligand-binding pocket of integrin alpha5beta1 using a gain-of-function approach.The Biochemical journal · 2009Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The overall structure of integrins is that of a ligand-binding head connected to two long legs. The legs can exhibit a pronounced bend at the "knees," and it has been proposed that the legs undergo a dramatic straightening when integrins transit from a low affinity to a high affinity state. The knee region contains domains from both alpha and beta subunits, including the N-terminal plexin/semaphorin/integrin (PSI) domain of the beta subunit. The role played by the knee domains in the regulation of integrin-ligand binding is uncertain. Here we show that: (i) monoclonal antibodies (mAbs) N29 and 8E3 have epitopes in the beta(1) subunit PSI domain and stimulate ligand binding to alpha(5)beta(1); (ii) N29 and 8E3 cause long range conformational changes that alter the ligand binding activity of the head region; (iii) the stimulatory action of these mAbs is dependent on the calf-1 domain, which forms part of the alpha subunit knee; and (iv) the epitopes of 8E3 and N29 map close to the extreme N terminus of the PSI and are likely to lie on the side of this domain that faces the alpha subunit. Taken together, our data suggest that the binding of these mAbs results in a levering apart of the PSI and calf-1 domains, and thereby causes the alpha and beta subunit knees to separate. Several major inferences can be drawn from our findings. First, the PSI domain appears to form part of an interface with the alpha subunit that normally restrains the integrin in a bent state. Second, the PSI domain is important for the transduction of conformational changes from the knee to head. Third, unbending is likely to provide a general mechanism for control of integrin-ligand recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.