ArticlePlant physiology2004
Identification and characterization of a Ca2+-dependent actin filament-severing protein from lily pollen.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 69 citations in OpenAlex.
- Alterations of actin in aging.Journal of cell science · 2026Review
- The quest for the central players governing pollen tube growth and guidance.Plant physiology · 2021Review
- Cytosolic calcium localization and dynamics during early endosperm development in the genus Agave (Asparagales, Asparagaceae).Protoplasma · 2019Article
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
- Arabidopsis FIM4 and FIM5 regulates the growth of root hairs in an auxin-insensitive way.Plant signaling & behavior · 2018Article
- Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes.Plant physiology · 2017Review
- ROS Regulation of Polar Growth in Plant Cells.Plant physiology · 2016Review
- Dual Roles of the Lysine-Rich Matrix Protein (KRMP)-3 in Shell Formation of Pearl Oyster, Pinctada fucata.PloS one · 2015Article
- Gelsolin-Like Domain 3 Plays Vital Roles in Regulating the Activities of the Lily Villin/Gelsolin/Fragmin Superfamily.PloS one · 2015Article
- Down-regulating annexin gene GhAnn2 inhibits cotton fiber elongation and decreases Ca2+ influx at the cell apex.Plant molecular biology · 2014Article
- Arabidopsis microtubule-destabilizing protein 25 functions in pollen tube growth by severing actin filaments.The Plant cell · 2014Article
- FIMBRIN1 is involved in lily pollen tube growth by stabilizing the actin fringe.The Plant cell · 2012Article
- RICE MORPHOLOGY DETERMINANT encodes the type II formin FH5 and regulates rice morphogenesis.The Plant cell · 2011Article
- Arabidopsis LIM proteins: a family of actin bundlers with distinct expression patterns and modes of regulation.The Plant cell · 2010Article
- Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover.The Plant cell · 2010Article
- The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division.The Plant cell · 2010Article
- Article
- Cloning and characterization of Prisilkin-39, a novel matrix protein serving a dual role in the prismatic layer formation from the oyster Pinctada fucata.The Journal of biological chemistry · 2009Article
- New findings in the mechanisms regulating polar growth in root hair cells.Plant signaling & behavior · 2009Review
- An actin-binding protein, LlLIM1, mediates calcium and hydrogen regulation of actin dynamics in pollen tubes.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well known that a tip-focused intracellular Ca2+ gradient and the meshwork of short actin filaments at the tip region are necessary for pollen tube growth. However, little is known about the connections between the two factors. Here, a novel Ca2+-dependent actin-binding protein with molecular mass of 41 kD from lily (Lilium davidii) pollen (LdABP41) was isolated and purified with DNase I chromatography. Our purification procedure yielded about 0.6 mg of LdABP41 with >98% purity from 10 g of lily pollen. At least two isoforms with isoelectric points of 5.8 and 6.0 were detected on two-dimensional gels. The results of N-terminal sequencing and mass-spectrometry analysis of LdABP41 showed that both isoforms shared substantial similarity with trumpet lily (Lilium longiflorum) villin and other members of the gelsolin superfamily. Negative-stained electron microscope images showed that LdABP41 severed in vitro-polymerized lily pollen F-actin into short actin filaments in a Ca2+-sensitive manner. Microinjection of the anti-LdABP41 antibody into germinated lily pollen demonstrated that the protein was required for pollen tube growth. The results of immunolocalization of the protein showed that it existed in the cytoplasm of the pollen tube, especially focused in the tip region. Our results suggest that LdABP41 belongs to the gelsolin superfamily and may play an important role in controlling actin organization in the pollen tube tip by responding to the oscillatory, tip-focused Ca2+ gradient.
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