ArticlePlant physiology2004
A green fluorescent protein fusion to actin-binding domain 2 of Arabidopsis fimbrin highlights new features of a dynamic actin cytoskeleton in live plant cells.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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Who cites it
127 citing papers in PubMed, 267 citations in OpenAlex.
- The Lipotubuloids ofPlants (Basel, Switzerland) · 2025Article
- Dynamics of full-length Arabidopsis myosin XI and its involvement in actin remodeling.Plant & cell physiology · 2025Article
- Functional fluorescence labeling of actins in live-cell system using engineered split-GFP technology.Molecular biology of the cell · 2025Article
- Identification, characterization and expression analysis of the CDPK family in kelp Saccharina japonica.BMC genomics · 2025Article
- Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration.Plant cell reports · 2025Article
- Structure of the F-tractin-F-actin complex.The Journal of cell biology · 2025Article
- Analytical methods for cytoplasmic streaming in elongated cells.PNAS nexus · 2025Article
- The genetic mechanism of B chromosome drive in rye illuminated by chromosome-scale assembly.Nature communications · 2024Article
- Pleiotropic regulatory locus 1 maintains actin cytoskeleton integrity and cellular homeostasis to enable Arabidopsis root growth.iScience · 2024Article
- Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments.Nature communications · 2024Article
- Disrupting cell wall integrity impacts endomembrane trafficking to promote secretion over endocytic trafficking.Journal of experimental botany · 2024Article
- Cooperative actin filament nucleation by the Arp2/3 complex and formins maintains the homeostatic cortical array in Arabidopsis epidermal cells.The Plant cell · 2024Article
- Detection of Stress-Induced Changes in Subcellular Protein Distribution.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cotton fiber as a model for understanding shifts in cell development under domestication.Frontiers in plant science · 2023Review
- Article
- Lipid Signaling Requires ROS Production to Elicit Actin Cytoskeleton Remodeling during Plant Innate Immunity.International journal of molecular sciences · 2022Article
- Imaging the living plant cell: From probes to quantification.The Plant cell · 2022Review
- Influencing the Actin Dynamics in Plant Cells by Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Methods to Visualize the Actin Cytoskeleton During Plant Cell Division.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
67 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cytoskeleton coordinates numerous cellular processes required for plant development. The functions of this network are intricately linked to its dynamic arrangement, and thus progress in understanding how actin orchestrates cellular processes relies on critical evaluation of actin organization and turnover. To investigate the dynamic nature of the actin cytoskeleton, we used a fusion protein between green fluorescent protein (GFP) and the second actin-binding domain (fABD2) of Arabidopsis (Arabidopsis thaliana) fimbrin, AtFIM1. The GFP-fABD2 fusion protein labeled highly dynamic and dense actin networks in diverse species and cell types, revealing structural detail not seen with alternative labeling methods, such as the commonly used mouse talin GFP fusion (GFP-mTalin). Further, we show that expression of the GFP-fABD2 fusion protein in Arabidopsis, unlike GFP-mTalin, has no detectable adverse effects on plant morphology or development. Time-lapse confocal microscopy and fluorescence recovery after photobleaching analyses of the actin cytoskeleton labeled with GFP-fABD2 revealed that lateral-filament migration and sliding of individual actin filaments or bundles are processes that contribute to the dynamic and continually reorganizing nature of the actin scaffold. These new observations of the dynamic actin cytoskeleton in plant cells using GFP-fABD2 reveal the value of this probe for future investigations of how actin filaments coordinate cellular processes required for plant development.
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