ArticlePharmaceutical research2004
Binding and uptake of wheat germ agglutinin-grafted PLGA-nanospheres by caco-2 monolayers.
Article in Pharmaceutical research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Wheat Germ Agglutinin-Conjugated Disulfide Cross-Linked Alginate Nanoparticles as a Docetaxel Carrier for Colon Cancer Therapy.International journal of nanomedicine · 2021Article
- Docetaxel-Loaded Disulfide Cross-Linked Nanoparticles Derived from Thiolated Sodium Alginate for Colon Cancer Drug Delivery.Pharmaceutics · 2020Article
- PLGA/xylitol nanoparticles enhance antibiofilm activityRSC advances · 2019Article
- Uptake and function of membrane-destabilizing cationic nanogels for intracellular drug delivery.Bioengineering & translational medicine · 2019Article
- Polymeric nanoparticle drug delivery technologies for oral delivery applications.Expert opinion on drug delivery · 2015Review
- Folic acid functionalized nanoparticles for enhanced oral drug delivery.Molecular pharmaceutics · 2012Article
- In vitro-in situ permeability and dissolution of fexofenadine with kinetic modeling in the presence of sodium dodecyl sulfate.European journal of drug metabolism and pharmacokinetics · 2012Article
- Biodegradable PLGA based nanoparticles for sustained regional lymphatic drug delivery.Journal of pharmaceutical sciences · 2010Article
- Ligand-modified gene carriers increased uptake in target cells but reduced DNA release and transfection efficiency.Nanomedicine : nanotechnology, biology, and medicine · 2010Article
- Wheat germ agglutinin functionalized complexation hydrogels for oral insulin delivery.Biomacromolecules · 2008Article
- Retrograde traffic in the biosynthetic-secretory route.Histochemistry and cell biology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe Caco-2 association of lectin-grafted PLGA-nanospheres was investigated compared to plain and BSA-coated spheres.
methodsNanospheres made from fluorescent-labeled PLGA were coated with wheat germ agglutinin (WGA) or BSA and incubated with Caco-2 monolayers varying the concentration of nanospheres, the time, and the temperature. The tests were performed in a static horizontal as well as an aerated vertical setup to find out the system most appropriate for estimation of bioadhesion.
resultsDue to bioadhesive effects, WGA-modified particles exhibited highest association to the cells as compared to plain and BSA-coated ones. The amount of associated spheres increased with time and concentration of the nanosphere suspension. Whereas the binding of lectin-coated spheres was independent from energy, their uptake was energy consuming as opposed to BSA and plain nanospheres, which exhibited nonspecific, energy independent binding and uptake. Although more particles were associated with the monolayer in the horizontal setup than in the vertical system, the vertical system reflects true bioadhesion due to circulation of the spheres which inhibits the influence of sedimentation.
conclusionsImmobilization of WGA considerably enhances the binding as well as the uptake of PLGA-nanospheres by Caco-2 monolayers. For bioadhesion studies, the vertical setup is recommended instead of the horizontal setup.
Indexed as
Identifiers
15553240What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.