ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
Two quality-associated HMW glutenin subunits in a somatic hybrid line between Triticum aestivum and Agropyron elongatum.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Asymmetric Somatic Hybridization Affects Synonymous Codon Usage Bias in Wheat.Frontiers in genetics · 2021Article
- The non-random patterns of genetic variation induced by asymmetric somatic hybridization in wheat.BMC plant biology · 2018Article
- A novel compensating wheat-Breeding science · 2017Article
- Article
- Genetic and epigenetic changes in somatic hybrid introgression lines between wheat and tall wheatgrass.Genetics · 2015Article
- The place of asymmetric somatic hybridization in wheat breeding.Plant cell reports · 2014Review
- Molecular cloning and variation of ω-gliadin genes from a somatic hybrid introgression line II-12 and parents (Triticum aestivum cv. Jinan 177 and Agropyron elongatum).Journal of genetics · 2011Article
- Article
- Generation of high frequency of novel alleles of the high molecular weight glutenin in somatic hybridization between bread wheat and tall wheatgrass.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Article
- Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Generation of novel high quality HMW-GS genes in two introgression lines of Triticum aestivum/Agropyron elongatum.BMC evolutionary biology · 2007Article
- LMW-GS genes in Agropyron elongatum and their potential value in wheat breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
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4 authors.
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Abstract
High-molecular-weight glutenin subunits (HMW-GSs) from hybrid line II-12 between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nivski were characterized with SDS-PAGE. Out of these HMW-GSs, two subunits, h1Bx and h1By, had mobilities similar to the subunits 1Bx13 and 1By16 from common wheat 4072, which was used as control. Polyclonal antibodies (pAbs) of h1Bx and h1By were prepared, and Western blotting showed that the pAbs had strong affinities for h1Bx and h1By, separately. The specificity of h1Bx-pAb was further checked; it preferentially recognized subunits h1Bx and 1Bx13. HMW-GS gene coding sequences were amplified by genomic polymerase chain reaction from hybrid II-12. Two of the five amplicons, marked II2a and II31b, were sequenced. Their coding sequences are clustered to Glu-1Bx7 and Glu-1By9 of common wheat. Three discrepant regions in deduced amino acid sequences of II2a and 31b repeated one time more than Glu-1Bx7 and Glu-1By9. N-terminal sequences of h1Bx and h1By were determined, which were identical to the published sequences of 1Bx13 and 1By16 and in agreement with that deduced from II2a and II31b, respectively. These results indicated that the two novel genes separated from the hybrid wheat derived from the allelic variation of 1Bx7 and 1By9 of the parent wheat. There is an additional cysteine residue positioned at 271st amino acid of the mature peptide of II2a, which may be related to the high quality of the flour.
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