ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Time-resolved measurement of state-specific P2X2 ion channel cytosolic gating motions.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- Finely ordered intracellular domain harbors an allosteric site to modulate physiopathological function of P2X3 receptors.Nature communications · 2024Article
- Dilation of ion selectivity filters in cation channels.Trends in biochemical sciences · 2024Review
- Enlightening activation gating in P2X receptors.Purinergic signalling · 2022Review
- On the permeation of large organic cations through the pore of ATP-gated P2X receptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Physical basis of apparent pore dilation of ATP-activated P2X receptor channels.Nature neuroscience · 2015Article
- Quantifying Ca2+ current and permeability in ATP-gated P2X7 receptors.The Journal of biological chemistry · 2015Article
- Role of the outer pore domain in transient receptor potential vanilloid 1 dynamic permeability to large cations.The Journal of biological chemistry · 2015Article
- Application of fluorescence resonance energy transfer in protein studies.Journal of molecular structure · 2014Article
- Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin.The Journal of general physiology · 2014Article
- Patch-clamp fluorometry: electrophysiology meets fluorescence.Biophysical journal · 2014Review
- Post-translational regulation of P2X receptor channels: modulation by phospholipids.Frontiers in cellular neuroscience · 2013Review
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- Emerging approaches to probing ion channel structure and function.Neuroscience bulletin · 2012Review
- Gating properties of the P2X2a and P2X2b receptor channels: experiments and mathematical modeling.The Journal of general physiology · 2012Article
- P2X2 and P2X5 subunits define a new heteromeric receptor with P2X7-like properties.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- A putative extracellular salt bridge at the subunit interface contributes to the ion channel function of the ATP-gated P2X2 receptor.The Journal of biological chemistry · 2010Article
- Dynamic aspects of functional regulation of the ATP receptor channel P2X2.The Journal of physiology · 2009Review
- Direct observation of ATP-induced conformational changes in single P2X(4) receptors.PLoS biology · 2009Article
- P2X receptors in sensory neurons co-cultured with cancer cells exhibit a decrease in opioid sensitivity.The European journal of neuroscience · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP-gated P2X2 channels undergo permeability changes through a process that is incompletely understood. In the present study, we used fluorescence resonance energy transfer (FRET) and electrophysiology to measure cytosolic gating motions in P2X2 channels as they enter a state with increased permeability. P2X2 channels underwent permeability changes with a time course that was similar to decreases in FRET between cyan fluorescent protein and yellow fluorescent protein attached to the cytosolic domain of P2X2 channels. Wild-type and mutant channels that did not undergo permeability changes also did not show evidence of cytosolic gating motions. Moreover, immobilizing the cytosolic domain by tethering it to the plasma membrane prevented the switch in permeability and impaired the cytosolic gating motions. Both of these phenotypes were restored when the immobilizing tether was cleaved. The data provide a time-resolved measure of state-specific gating motions and suggest how a cytosolic domain may control ion channel permeability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.