ArticleMolecular biology of the cell2005
Cdc42 mediates nucleus movement and MTOC polarization in Swiss 3T3 fibroblasts under mechanical shear stress.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 94 citations in OpenAlex.
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- Endothelial cells on the move: dynamics in vascular morphogenesis and disease.Vascular biology (Bristol, England) · 2020Review
- Recapitulation of molecular regulators of nuclear motion during cell migration.Cell adhesion & migration · 2019Review
- The Interplay Between Cell-Cell and Cell-Matrix Forces Regulates Cell Migration Dynamics.Biophysical journal · 2019Article
- Perioperative implication of the endothelial glycocalyx.Korean journal of anesthesiology · 2018Review
- Integrating transient cellular and nuclear motions to comprehensively describe cell migration patterns.Scientific reports · 2018Article
- Corneal epithelial cells exposed to shear stress show altered cytoskeleton and migratory behaviour.PloS one · 2017Article
- Displacement correlations between a single mesenchymal-like cell and its nucleus effectively link subcellular activities and motility in cell migration analysis.Scientific reports · 2016Article
- The Mechanobiology of Aging.Annual review of biomedical engineering · 2015Review
- Coherent motions in confluent cell monolayer sheets.Biophysical journal · 2014Article
- Tight coupling between nucleus and cell migration through the perinuclear actin cap.Journal of cell science · 2014Article
- Actomyosin contractility rotates the cell nucleus.Scientific reports · 2014Article
- Article
- Emerging role for nuclear rotation and orientation in cell migration.Cell adhesion & migration · 2014Article
- Nuclear envelope in nuclear positioning and cell migration.Advances in experimental medicine and biology · 2014Review
- Cyclooxygenase-2-derived prostaglandin E₂ promotes injury-induced vascular neointimal hyperplasia through the E-prostanoid 3 receptor.Circulation research · 2013Article
- The multi-faceted role of the actin cap in cellular mechanosensation and mechanotransduction.Soft matter · 2013Article
- Focal adhesion size uniquely predicts cell migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- The nucleus of endothelial cell as a sensor of blood flow direction.Biology open · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleus movement is essential during nucleus positioning for tissue growth and development in eukaryotic cells. However, molecular regulators of nucleus movement in interphase fibroblasts have yet to be identified. Here, we report that nuclei of Swiss 3T3 fibroblasts undergo enhanced movement when subjected to shear flows. Such movement includes both rotation and translocation and is dependent on microtubule, not F-actin, structure. Through inactivation of Rho GTPases, well-known mediators of cytoskeleton reorganization, we demonstrate that Cdc42, not RhoA or Rac1, controls the extent of nucleus translocation, and more importantly, of nucleus rotation in the cytoplasm. In addition to generating nuclei movement, we find that shear flows also causes repositioning of the MTOC in the direction of flow. This behavior is also controlled by Cdc42 via the Par6/protein kinase Czeta pathway. These results are the first to establish Cdc42 as a molecular regulator of not only shear-induced MTOC polarization in Swiss 3T3 fibroblasts, but also of shear-induced microtubule-dependent nucleus movement. We propose that the movements of MTOC and nucleus are coupled chemically, because they are both regulated by Cdc42 and dependent on microtubule structure, and physically, possibly via Hook/SUN family homologues similar to those found in Caenorhabditis elegans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.