ArticleMolecular biology of the cell2005
The role of microtubules in rapid hyphal tip growth of Aspergillus nidulans.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 185 citations in OpenAlex.
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- Secretion and endocytosis in subapical cells support hyphal tip growth in the fungus Trichoderma reesei.Nature communications · 2025Article
- The Microtubule End Binding Protein Mal3 Is Essential for the Dynamic Assembly of Microtubules duringInternational journal of molecular sciences · 2024Article
- Targeting of Specialized Metabolites Biosynthetic Enzymes to Membranes and Vesicles by Posttranslational Palmitoylation: A Mechanism of Non-Conventional Traffic and Secretion of Fungal Metabolites.International journal of molecular sciences · 2024Review
- Aspergillus SUMOylation mutants exhibit chromosome segregation defects including chromatin bridges.Genetics · 2023Article
- Cell wall dynamics stabilize tip growth in a filamentous fungus.PLoS biology · 2023Article
- Mannitol-1-phosphate dehydrogenase, MpdA, is required for mannitol production in vegetative cells and involved in hyphal branching, heat resistance of conidia and sexual development in Aspergillus nidulans.Current genetics · 2021Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Application of PALM Superresolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Translocation of nutrient transporters to cell membrane via Golgi bypass in Aspergillus nidulans.EMBO reports · 2020Article
- Transport systems, intracellular traffic of intermediates and secretion of β-lactam antibiotics in fungi.Fungal biology and biotechnology · 2020Review
- The Dis1/Stu2/XMAP215 Family GeneFrontiers in microbiology · 2020Article
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli.Molecular biology of the cell · 2019Article
- Nup2 performs diverse interphase functions in Aspergillus nidulans.Molecular biology of the cell · 2018Article
- Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures.Microbiology and molecular biology reviews : MMBR · 2018Review
- Superresolution and pulse-chase imaging reveal the role of vesicle transport in polar growth of fungal cells.Science advances · 2018Article
- Horizontal Gene Transfer From Bacteria and Plants to the Arbuscular Mycorrhizal FungusFrontiers in plant science · 2018Article
- Location and functional analysis of the Aspergillus nidulans Aurora kinase confirm mitotic functions and suggest non-mitotic roles.Fungal genetics and biology : FG & B · 2017Article
- Pulses of CaProceedings of the National Academy of Sciences of the United States of America · 2017Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
The filamentous fungus Aspergillus nidulans grows by polarized extension of hyphal tips. The actin cytoskeleton is essential for polarized growth, but the role of microtubules has been controversial. To define the role of microtubules in tip growth, we used time-lapse microscopy to measure tip growth rates in germlings of A. nidulans and in multinucleate hyphal tip cells, and we used a green fluorescent protein-alpha-tubulin fusion to observe the effects of the antimicrotubule agent benomyl. Hyphal tip cells grew approximately 5 times faster than binucleate germlings. In germlings, cytoplasmic microtubules disassembled completely in mitosis. In hyphal tip cells, however, microtubules disassembled through most of the cytoplasm in mitosis but persisted in a region near the hyphal tip. The growth rate of hyphal tip cells did not change significantly in mitosis. Benomyl caused rapid disassembly of microtubules in tip cells and a 10x reduction in growth rate. When benomyl was washed out, microtubules assembled quickly and rapid tip growth resumed. These results demonstrate that although microtubules are not strictly required for polarized growth, they are rate-limiting for the growth of hyphal tip cells. These data also reveal that A. nidulans exhibits a remarkable spatial regulation of microtubule disassembly within hyphal tip cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.