ArticlePLoS biology2004
Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets.
Article in PLoS biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
141 citing papers in PubMed, 322 citations in OpenAlex.
- RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1.Nucleic acids research · 2026Article
- Semi-automated Detection of Late Cytokinetic Bridges.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Fission yeast Duc1 links to ER-PM contact sites and influences PM lipid composition and cytokinetic ring anchoring.Journal of cell science · 2024Article
- Vesicle-mediated transport of ALIX and ESCRT-III to the intercellular bridge during cytokinesis.Cellular and molecular life sciences : CMLS · 2023Article
- An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis.Nature communications · 2023Article
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Lipid Polarization during Cytokinesis.Cells · 2022Review
- Article
- A Ruthenium(II) Polypyridyl Complex Disrupts Actin Cytoskeleton Assembly and Blocks Cytokinesis.Angewandte Chemie (International ed. in English) · 2022Article
- A Ruthenium(II) Polypyridyl Complex Disrupts Actin Cytoskeleton Assembly and Blocks Cytokinesis.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- The microtubule- and PP1-binding activities ofMolecular biology of the cell · 2022Article
- Multiple pools of PP2A regulate spindle assembly, kinetochore attachments and cohesion in Drosophila oocytes.Journal of cell science · 2021Article
- Image-based profiling for drug discovery: due for a machine-learning upgrade?Nature reviews. Drug discovery · 2021Review
- Hyperpolyploidization of hepatocyte initiates preneoplastic lesion formation in the liver.Nature communications · 2021Article
- Moonlighting in Mitosis: Analysis of the Mitotic Functions of Transcription and Splicing Factors.Cells · 2020Review
- The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis.Nature communications · 2020Article
- Membrane and organelle dynamics during cell division.Nature reviews. Molecular cell biology · 2020Review
- A simple and robust cell-based assay for the discovery of novel cytokinesis inhibitors.Journal of biological methods · 2020Article
- RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.PLoS genetics · 2019Article
- Review
81 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Cytokinesis involves temporally and spatially coordinated action of the cell cycle and cytoskeletal and membrane systems to achieve separation of daughter cells. To dissect cytokinesis mechanisms it would be useful to have a complete catalog of the proteins involved, and small molecule tools for specifically inhibiting them with tight temporal control. Finding active small molecules by cell-based screening entails the difficult step of identifying their targets. We performed parallel chemical genetic and genome-wide RNA interference screens in Drosophila cells, identifying 50 small molecule inhibitors of cytokinesis and 214 genes important for cytokinesis, including a new protein in the Aurora B pathway (Borr). By comparing small molecule and RNAi phenotypes, we identified a small molecule that inhibits the Aurora B kinase pathway. Our protein list provides a starting point for systematic dissection of cytokinesis, a direction that will be greatly facilitated by also having diverse small molecule inhibitors, which we have identified. Dissection of the Aurora B pathway, where we found a new gene and a specific small molecule inhibitor, should benefit particularly. Our study shows that parallel RNA interference and small molecule screening is a generally useful approach to identifying active small molecules and their target pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.