ArticleJournal of bacteriology2004
Negative transcriptional regulation of the ilv-leu operon for biosynthesis of branched-chain amino acids through the Bacillus subtilis global regulator TnrA.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Coenzyme A biosynthesis inmBio · 2024Article
- Microbial synthesis of bacitracin: Recent progress, challenges, and prospects.Synthetic and systems biotechnology · 2023Review
- Bioinformatics Modelling and Metabolic Engineering of the Branched Chain Amino Acid Pathway for Specific Production of Mycosubtilin Isoforms inMetabolites · 2022Article
- Transcriptomic and Phenotypic Analyses of the Sigma B-Dependent Characteristics and the Synergism between Sigma B and Sigma L inMicroorganisms · 2020Article
- Article
- TheApplied and environmental microbiology · 2019Article
- Genome-wide mapping of TnrA-binding sites provides new insights into the TnrA regulon in Bacillus subtilis.MicrobiologyOpen · 2015Article
- Regulating the Intersection of Metabolism and Pathogenesis in Gram-positive Bacteria.Microbiology spectrum · 2015Review
- CcpA-mediated catabolite activation of the Bacillus subtilis ilv-leu operon and its negation by either CodY- or TnrA-mediated negative regulation.Journal of bacteriology · 2014Article
- Comparative genome analysis of central nitrogen metabolism and its control by GlnR in the class Bacilli.BMC genomics · 2012Article
- The essentiality of staphylococcal Gcp is independent of its repression of branched-chain amino acids biosynthesis.PloS one · 2012Article
- Rapid changes in gene expression: DNA determinants of promoter regulation by the concentration of the transcription initiating NTP in Bacillus subtilis.Nucleic acids research · 2011Article
- Transcriptional de-repression and Mfd are mutagenic in stressed Bacillus subtilis cells.Journal of molecular microbiology and biotechnology · 2011Article
- Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools.Journal of bacteriology · 2010Article
- Time-resolved transcriptome analysis of Bacillus subtilis responding to valine, glutamate, and glutamine.PloS one · 2009Article
- Regulation of Bacillus subtilis aprE expression by glnA through inhibition of scoC and sigma(D)-dependent degR expression.Journal of bacteriology · 2009Article
- Molecular mechanisms underlying the positive stringent response of the Bacillus subtilis ilv-leu operon, involved in the biosynthesis of branched-chain amino acids.Journal of bacteriology · 2008Article
- Cross-regulation of the Bacillus subtilis glnRA and tnrA genes provides evidence for DNA binding site discrimination by GlnR and TnrA.Journal of bacteriology · 2006Article
- Intracellular gene expression profile of Listeria monocytogenes.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bacillus subtilis ilv-leu operon is involved in the synthesis of branched-chain amino acids (valine, isoleucine, and leucine). The two- to threefold repression of expression of the ilv-leu operon during logarithmic-phase growth under nitrogen-limited conditions, which was originally detected by a DNA microarray analysis to compare the transcriptomes from the wild-type and tnrA mutant strains, was confirmed by lacZ fusion and Northern experiments. A genome-wide TnrA box search revealed a candidate box approximately 200 bp upstream of the transcription initiation base of the ilv-leu operon, the TnrA binding to which was verified by gel retardation and DNase I footprinting analyses. Deletion and base substitution of the TnrA box sequence affected the ilv-leu promoter activity in vivo, implying that TnrA bound to the box might be able to inhibit the promoter activity, possibly through DNA bending. The negative control of the expression of the ilv-leu operon by TnrA, which is considered to represent rather fine-tuning (two- to threefold), is a novel regulatory link between nitrogen and amino acid metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.