ArticleNucleic acids research2004
Negatively charged self-assembling DNA/poloxamine nanospheres for in vivo gene transfer.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 86 citations in OpenAlex.
- 704/DNA vaccines leverage cytoplasmic DNA stimulation to promote anti-HIV neutralizing antibody production in mice and strong immune response against alpha-fetoprotein in non-human primates.Molecular therapy. Nucleic acids · 2023Article
- Conventional and Nanotechnology Based Approaches to Combat Chronic Obstructive Pulmonary Disease: Implications for Chronic Airway Diseases.International journal of nanomedicine · 2020Review
- The proper strategy to compress and protect plasmid DNA in the Pluronic L64-electropulse system for enhanced intramuscular gene delivery.Regenerative biomaterials · 2019Article
- Tetrafunctional Block Copolymers Promote Lung Gene Transfer in Newborn Piglets.Molecular therapy. Nucleic acids · 2019Article
- Self-assembled peptide-poloxamine nanoparticles enable in vitro and in vivo genome restoration for cystic fibrosis.Nature nanotechnology · 2019Article
- Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes.International journal of nanomedicine · 2015Article
- Block copolymer/DNA vaccination induces a strong allergen-specific local response in a mouse model of house dust mite asthma.PloS one · 2014Article
- 2-diethylaminoethyl-dextran methyl methacrylate copolymer nonviral vector: still a long way toward the safety of aerosol gene therapy.Gene therapy · 2013Article
- Osteoprotegerin inhibits bone resorption and prevents tumor development in a xenogenic model of Ewing's sarcoma by inhibiting RANKL.Journal of bone oncology · 2013Article
- Poly(alkylene oxide) copolymers for nucleic acid delivery.Accounts of chemical research · 2012Article
- Formulation and characterization of poloxamine-based hydrogels as tissue sealants.Acta biomaterialia · 2012Article
- Vectors for inhaled gene therapy in lung cancer. Application for nano oncology and safety of bio nanotechnology.International journal of molecular sciences · 2012Review
- Effect of fractalkine-Fc delivery in experimental lung metastasis using DNA/704 nanospheres.Cancer gene therapy · 2011Article
- Amphiphilic block copolymers enhance the cellular uptake of DNA molecules through a facilitated plasma membrane transport.Nucleic acids research · 2011Article
- Cationic surface modification of PLG nanoparticles offers sustained gene delivery to pulmonary epithelial cells.Journal of pharmaceutical sciences · 2010Article
- Block copolymers have differing adjuvant effects on the primary immune response elicited by genetic immunization and on further induced allergy.Clinical and vaccine immunology : CVI · 2010Article
- DNA/amphiphilic block copolymer nanospheres promote low-dose DNA vaccination.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Therapeutic efficacy of soluble receptor activator of nuclear factor-kappa B-Fc delivered by nonviral gene transfer in a mouse model of osteolytic osteosarcoma.Molecular cancer therapeutics · 2008Article
- Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Heart-specific inhibition of protooncogene c-myc attenuates cold-induced cardiac hypertrophy.Gene therapy · 2007Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past decade, numerous nonviral cationic vectors have been synthesized. They share a high density of positive charges and efficiency for gene transfer in vitro. However, their positively charged surface causes instability in body fluids and cytotoxicity, thereby limiting their efficacy in vivo. Therefore, there is a need for developing alternative molecular structures. We have examined tetrabranched amphiphilic block copolymers consisting of four polyethyleneoxide/polypropyleneoxide blocks centered on an ethylenediamine moiety. Cryo-electron microscopy, ethidium bromide fluorescence and light and X-ray scattering experiments performed on vector-DNA complexes showed that the dense core of the nanosphere consisted of condensed DNA interacting with poloxamine molecules through electrostatic, hydrogen bonding and hydrophobic interactions, with DNA molecules also being exposed at the surface. The supramolecular organization of block copolymer/DNA nanospheres induced the formation of negatively charged particles. These particles were stable in a solution that had a physiological ionic composition and were resistant to decomplexation by heparin. The new nanostructured material, the structure of which clearly contrasted with that of lipoplexes and polyplexes, efficiently transferred reporter and therapeutic genes in skeletal and heart muscle in vivo. Negatively charged supramolecular assemblies hold promise as therapeutic gene carriers for skeletal and heart muscle-related diseases and expression of therapeutic proteins for local or systemic uses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.