ArticleRNA (New York, N.Y.)2004
Nonsense-mediated decay does not occur within the yeast nucleus.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Pseudouridine-mediated stop codon readthrough inRNA (New York, N.Y.) · 2020Article
- Cap-binding complex (CBC).The Biochemical journal · 2014Review
- Competition between pre-mRNAs for the splicing machinery drives global regulation of splicing.Molecular cell · 2013Article
- Nonsense-mediated mRNA decay occurs during eIF4F-dependent translation in human cells.Nature structural & molecular biology · 2013Article
- Ty1 gag enhances the stability and nuclear export of Ty1 mRNA.Traffic (Copenhagen, Denmark) · 2013Article
- Loss of nonsense mediated decay suppresses mutations in Saccharomyces cerevisiae TRA1.BMC genetics · 2012Article
- Yeast mRNA cap-binding protein Cbc1/Sto1 is necessary for the rapid reprogramming of translation after hyperosmotic shock.Molecular biology of the cell · 2012Article
- Article
- Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe.The EMBO journal · 2010Article
- Nuclear expression of a group II intron is consistent with spliceosomal intron ancestry.Genes & development · 2010Article
- Nonsense-mediated mRNA decay in human cells: mechanistic insights, functions beyond quality control and the double-life of NMD factors.Cellular and molecular life sciences : CMLS · 2010Review
- Nonsense-mediated mRNA decay (NMD) mechanisms.Nature structural & molecular biology · 2009Article
- Conventional 3' end formation is not required for NMD substrate recognition in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2006Article
- Drosophila ribosomal proteins are associated with linker histone H1 and suppress gene transcription.Genes & development · 2006Article
- Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay.EMBO reports · 2006Article
- Systems-level analyses identify extensive coupling among gene expression machines.Molecular systems biology · 2006Article
- Association of ribosomal proteins with nascent transcripts in S. cerevisiae.RNA (New York, N.Y.) · 2005Article
- Transcript selection and the recruitment of mRNA decay factors for NMD in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2005Review
- Cap-binding protein 1-mediated and eukaryotic translation initiation factor 4E-mediated pioneer rounds of translation in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- "Dark matter" worlds of unstable RNA and protein.Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Nonsense-mediated decay (NMD) is a eukaryotic regulatory process that degrades mRNAs with premature termination codons (PTCs). Although NMD is a translation-dependent process, there is evidence from mammalian systems that PTC recognition and mRNA degradation takes place in association with nuclei. Consistent with this notion, degradation of mammalian PTC-containing mRNAs occurs when they are bound by the cap binding complex (CBC) during a "pioneer" round of translation. Moreover, there are reports indicating that a PTC can trigger other nuclear events such as alternative splicing, abnormal 3' end processing, and accumulation of pre-mRNA at transcription sites. To examine whether a PTC can elicit similar nuclear events in yeast, we used RNA export-defective mutants to sequester mRNAs within nuclei. The results indicate that nuclear PTC-containing yeast RNAs are NMD insensitive. We also observed by fluorescent in situ hybridization that there was no PTC effect on mRNA accumulated at the site of transcription. Finally, we show that yeast NMD occurs minimally if at all on CBC-bound transcripts, arguing against a CBC-mediated pioneer round of translation in yeast. The data taken together indicate that there are no direct consequences of a PTC within the yeast nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.