ArticleProceedings of the National Academy of Sciences of the United States of America2004
Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Ribosome External Electric Field Regulates Metabolic Enzyme Activity: The RAMBO Effect.The journal of physical chemistry. B · 2024Article
- Phosphorylation-Driven Epichaperome Assembly: A Critical Regulator of Cellular Adaptability and Proliferation.Research square · 2024Article
- Ribosome-Amplified Metabolism, RAMBO, Measured by NMR Spectroscopy.Biochemistry · 2021Article
- Evaluating the performance of an ETD-cleavable cross-linking strategy for elucidating protein structures.Journal of proteomics · 2020Article
- Allosteric Regulation of Rod Photoreceptor Phosphodiesterase 6 (PDE6) Elucidated by Chemical Cross-Linking and Quantitative Mass Spectrometry.Journal of molecular biology · 2019Article
- Mutagenesis of DsbAss is Crucial for the Signal Recognition Particle Mechanism inBiomolecules · 2019Article
- Chemical cross-linking in the structural analysis of protein assemblies.Methods (San Diego, Calif.) · 2018Review
- Co-evolution of Two GTPases Enables Efficient Protein Targeting in an RNA-less Chloroplast Signal Recognition Particle Pathway.The Journal of biological chemistry · 2017Article
- Change in the Molecular Dimension of a RAGE-Ligand Complex Triggers RAGE Signaling.Structure (London, England : 1993) · 2016Article
- ECL: an exhaustive search tool for the identification of cross-linked peptides using whole database.BMC bioinformatics · 2016Article
- Molecular architecture of photoreceptor phosphodiesterase elucidated by chemical cross-linking and integrative modeling.Journal of molecular biology · 2014Article
- Elucidating the mechanism of substrate recognition by the bacterial Hsp90 molecular chaperone.Journal of molecular biology · 2014Article
- Chemical crosslinkers enhance detection of receptor interactomes.Frontiers in pharmacology · 2014Review
- Conformational States of macromolecular assemblies explored by integrative structure calculation.Structure (London, England : 1993) · 2013Article
- Protein interactions, post-translational modifications and topologies in human cells.Molecular & cellular proteomics : MCP · 2013Article
- Mapping the structural topology of the yeast 19S proteasomal regulatory particle using chemical cross-linking and probabilistic modeling.Molecular & cellular proteomics : MCP · 2012Article
- Integrating mass spectrometry of intact protein complexes into structural proteomics.Proteomics · 2012Review
- Probing the conformation of the ISWI ATPase domain with genetically encoded photoreactive crosslinkers and mass spectrometry.Molecular & cellular proteomics : MCP · 2012Article
- Subunit order of eukaryotic TRiC/CCT chaperonin by cross-linking, mass spectrometry, and combinatorial homology modeling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Xlink-identifier: an automated data analysis platform for confident identifications of chemically cross-linked peptides using tandem mass spectrometry.Journal of proteome research · 2011Article
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8 authors.
Funding
Abstract
Among the methods used to unravel protein interaction surfaces, chemical cross-linking followed by identification of the cross-linked peptides by mass spectrometry has proven especially useful in dynamic and complex systems. During the signal recognition particle (SRP)-dependent targeting of proteins to the bacterial plasma membrane, the specific interaction between Ffh (the protein component of SRP) and FtsY (the SRP receptor) is known to be essential for the efficiency and fidelity of this process. In this work, we studied the Escherichia coli and Thermus aquaticus Ffh.FtsY complexes by using chemical cross-linking and tandem mass spectrometry to identify nine intermolecular cross-linked peptides. This information was used in conjunction with a previously undescribed model-building approach that combines geometric restraint optimization with macromolecular docking. The resulting model of the Ffh.FtsY complex is in good agreement with the crystal structure solved shortly thereafter. Intriguingly, four of the cross-linked pairs involve the M domain of Ffh, which is absent from the crystal structure, providing previously undocumented experimental evidence that the M domain is positioned in close proximity to the Ffh.FtsY interface in the complex.
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