ArticleMarine biotechnology (New York, N.Y.)
Suppression subtractive hybridization cDNA libraries to identify differentially expressed genes from contrasting fish habitats.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 26 citations in OpenAlex.
- Specific Evolution and Gene Family Expansion of Complement 3 and Regulatory Factor H in Fish.Frontiers in immunology · 2020Article
- Phytoestrogens modulate hepcidin expression by Nrf2: Implications for dietary control of iron absorption.Free radical biology & medicine · 2015Article
- Review
- Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions.Environmental science and pollution research international · 2014Article
- The mining of pearl formation genes in pearl oyster Pinctada fucata by cDNA suppression subtractive hybridization.Marine biotechnology (New York, N.Y.) · 2012Article
- Molecular identification and expression of differentially regulated genes of the European flounder, Platichthys flesus, submitted to pesticide exposure.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Suppression subtractive hybridization complementary DNA libraries identified differentially expressed genes in liver tissue of winter flounder collected from the highly impacted Raritan-Hudson estuary versus those from less industrialized estuaries farther south in New Jersey. Distinct transcript profiles emerged in the fish from these different habitats. A total of 251 clones from the forward (upregulated with anthropogenic impact) and reverse (downregulated with anthropogenic impact) subtracted libraries were sequenced. In the upregulated library immune response transcripts, including complement C-3, C-7, factor H, factor Bf/C2, differentially regulated trout protein 1, and the antimicrobial hepcidin, indicated the pollution-impacted fish were under a high viral or bacterial load. Transcripts for cytochrome P450 1A, P450 3A, and glutathione S-transferase, important components of phase I and II metabolism of xenobiotics, were found in the upregulated-with-pollution library. Vitellogenins I and II and egg envelope protein (zp) appeared to be downregulated. A homologue of the tumor suppressor p33(ING1) (down) and hepatocyte growth factor-like protein (up) may indicate liver damage or hepatocellular carcinoma or hepatoma. These expression patterns, confirmed by quantitative polymerase chain reaction, indicate that transcript analysis is a useful method for assessing the health of local habitats and the organisms therein.
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Registered trials
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