ArticleEndocrine2004
Comparison of metabolic oscillations from mouse pancreatic beta cells and islets.
Article in Endocrine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- CaAmerican journal of physiology. Endocrinology and metabolism · 2023Review
- Intact pancreatic islets and dispersed beta-cells both generate intracellular calcium oscillations but differ in their responsiveness to glucose.Cell calcium · 2019Article
- Beta cell connectivity in pancreatic islets: a type 2 diabetes target?Cellular and molecular life sciences : CMLS · 2015Review
- Episodic hormone secretion: a comparison of the basis of pulsatile secretion of insulin and GnRH.Endocrine · 2014Review
- Direct measurements of oscillatory glycolysis in pancreatic islet β-cells using novel fluorescence resonance energy transfer (FRET) biosensors for pyruvate kinase M2 activity.The Journal of biological chemistry · 2013Article
- Slow oscillations of KATP conductance in mouse pancreatic islets provide support for electrical bursting driven by metabolic oscillations.American journal of physiology. Endocrinology and metabolism · 2013Article
- Complex patterns of metabolic and Ca²⁺ entrainment in pancreatic islets by oscillatory glucose.Biophysical journal · 2013Article
- Plasma membrane potential oscillations in insulin secreting Ins-1 832/13 cells do not require glycolysis and are not initiated by fluctuations in mitochondrial bioenergetics.The Journal of biological chemistry · 2012Article
- Nifedipine protects INS-1 β-cell from high glucose-induced ER stress and apoptosis.International journal of molecular sciences · 2011Article
- Bursting and calcium oscillations in pancreatic beta-cells: specific pacemakers for specific mechanisms.American journal of physiology. Endocrinology and metabolism · 2010Review
- Metabolic oscillations in pancreatic islets depend on the intracellular Ca2+ level but not Ca2+ oscillations.Biophysical journal · 2010Article
- Glucose sensing in the pancreatic beta cell: a computational systems analysis.Theoretical biology & medical modelling · 2010Article
- Glucose metabolism, islet architecture, and genetic homogeneity in imprinting of [Ca2+](i) and insulin rhythms in mouse islets.PloS one · 2009Article
- Evidence of diminished glucose stimulation and endoplasmic reticulum function in nonoscillatory pancreatic islets.Endocrinology · 2009Article
- Glucose modulates [Ca2+]i oscillations in pancreatic islets via ionic and glycolytic mechanisms.Biophysical journal · 2006Article
- Calcium and Metabolic Oscillations in Pancreatic Islets: Who's Driving the Bus?SIAM journal on applied dynamical systemsArticle
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rhythmic insulin secretion from pancreatic islets is the culmination of many processes both intrinsic and extrinsic to the beta cell. We wished to examine and compare endogenous metabolic oscillations in islets and isolated single beta cells that underlie secretion. Fluorescence patterns of rhodamine 123, an indicator of mitochondrial membrane potential (DeltaPsim), were analyzed for period and amplitude of oscillations using two methods: CLUSTER7 and fast Fourier transform (FFT). The period of DeltaPsim oscillations was greater in islets (271 +/- 21 s, n = 34) compared to dispersed beta cells (180 +/- 10 s, n = 54) by FFT analysis (p < 0.0005). CLUSTER7 confirmed differences in period and also detected oscillatory amplitude differences between beta cells (12.0 +/- 0.8) and islets (7.1 +/- 2.3% of baseline fluorescence, p < 0.0001). Depolarizing responses to the mitochondrial poison NaN3 were reduced in beta cells (35 +/- 4%) vs islets (58 +/- 9%), and hyperpolarizing responses to the calcium channel blocker nifedipine were enhanced in beta cells (15 +/- 4%) vs islets (8 +/- 1%), suggesting that mitochondria in dispersed beta cells were less energized than those in intact islets (p < 0.005), possibly due to elevated intracellular calcium. These findings suggest that individual beta cells possess the proper machinery to generate metabolic oscillations. Differences in oscillatory period, DeltaPsim, and nifedipine response suggest that incorporation into islets provides beta cells with additional modulatory influences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.