ArticleGenes & development2004
Cold shock and regulation of surface protein trafficking convey sensitization to inducers of stage differentiation in Trypanosoma brucei.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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104 citing papers in PubMed.
- Glycerol metabolism triggers trypanosome differentiation into transmissible forms in mammalian tissue-like conditions.Nature communications · 2026Article
- The Dual Role of the 16mer Motif Within the 3' Untranslated Region of the Variant Surface Glycoprotein of Trypanosoma brucei.Molecular microbiology · 2026Article
- Thermo-related degeneration of stumpy forms of Trypanosoma brucei, the pathogen of African sleeping sickness.Advanced biotechnology · 2025Article
- A murine model ofMicrobiology spectrum · 2025Article
- Article
- The actomyosin system is essential for the integrity of the endosomal system in bloodstream formeLife · 2024Article
- Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes.eLife · 2024Article
- Q586B2 is a crucial virulence factor during the early stages of Trypanosoma brucei infection that is conserved amongst trypanosomatids.Nature communications · 2024Article
- The developmental hierarchy and scarcity of replicative slender trypanosomes in blood challenges their role in infection maintenance.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Metabolic insights into phosphofructokinase inhibition in bloodstream-form trypanosomes.Frontiers in cellular and infection microbiology · 2023Article
- Molecular Analysis of Trypanosome Infections in Algerian Camels.Acta parasitologica · 2022Article
- Organization of minicircle cassettes and guide RNA genes inRNA (New York, N.Y.) · 2022Article
- Article
- A long noncoding RNA promotes parasite differentiation in African trypanosomes.Science advances · 2022Article
- Article
- Excreted Trypanosoma brucei proteins inhibit Plasmodium hepatic infection.PLoS neglected tropical diseases · 2021Article
- Single-cell transcriptomic analysis of bloodstream Trypanosoma brucei reconstructs cell cycle progression and developmental quorum sensing.Nature communications · 2021Article
- p67: a cryptic lysosomal hydrolase inParasitology · 2021Article
- Trypanosoma brucei triggers a broad immune response in the adipose tissue.PLoS pathogens · 2021Article
44 more citing papers are in PubMed but not listed here.
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Transmission of a protozoan parasite from a vertebrate to invertebrate host is accompanied by cellular differentiation. The signals from the environment that trigger the process are poorly understood. The model parasite Trypanosoma brucei proliferates in the mammalian bloodstream and in the tsetse fly. On ingestion by the tsetse, the trypanosome undergoes a rapid differentiation that is marked by replacement of the variant surface glycoprotein (VSG) coat with GPI-anchored EP and GPEET procyclins. Here we show that a cold shock of DeltaT > 15 degrees C is sufficient to reversibly induce high-level expression of the insect stage-specific EP gene in the mammalian bloodstream stages of T. brucei. The 3'-UTR of the EP mRNA is necessary and sufficient for the increased expression. During cold shock, EP protein accumulates in the endosomal compartment in the proliferating, slender, bloodstream stage, whereas the EP is present on the plasma membrane in the quiescent, stumpy, bloodstream stage. Thus, there is a novel developmentally regulated cell surface access control mechanism for a GPI-anchored protein. In addition to inducing EP expression, cold shock results in the acquisition of sensitivity to micromolar concentrations of cis-aconitate and citrate by stumpy but not slender bloodstream forms. The cis-aconitate and citrate commit stumpy bloodstream cells to differentiation to the procyclic stage along with rapid initial proliferation. We propose a hierarchical model of three events that regulate differentiation after transmission to the tsetse: sensing the temperature change, surface access of a putative receptor, and sensing of a chemical cue.
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