ArticleClinical and experimental immunology2004
Cl- channels are expressed in human normal monocytes: a functional role in migration, adhesion and volume change.
Article in Clinical and experimental immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- FRET-based reporter assesses lysosomal DNA-degradation ability in live cells.Sensors and actuators reports · 2025Article
- Article
- Tunable Cytosolic Chloride Indicators for Real-Time Chloride Imaging in Live Cells.bioRxiv : the preprint server for biology · 2024Article
- Monocyte distribution width (MDW) as an infection indicator in severe patients attending in the Emergency Department: a pilot study.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2023Observational
- Monocyte Distribution Width, Neutrophil-to-Lymphocyte Ratio, and Platelet-to-Lymphocyte Ratio Improves Early Prediction for Sepsis at the Emergency.Journal of personalized medicine · 2021Article
- Leukocyte cell population data from the blood cell analyzer as a predictive marker for severity of acute pancreatitis.Journal of clinical laboratory analysis · 2021Article
- Monocyte Distribution Width: A Novel Indicator of Sepsis-2 and Sepsis-3 in High-Risk Emergency Department Patients.Critical care medicine · 2019Article
- Overexpression of chloride channel-3 (ClC-3) is associated with human cervical carcinoma development and prognosis.Cancer cell international · 2019Article
- Dynamic properties of calcium-activated chloride currents in Xenopus laevis oocytes.Scientific reports · 2017Article
- CLIC1 regulates dendritic cell antigen processing and presentation by modulating phagosome acidification and proteolysis.Biology open · 2016Article
- Chloride Channel 3 Channels in the Activation and Migration of Human Blood Eosinophils in Allergic Asthma.American journal of respiratory cell and molecular biology · 2015Article
- Intermediate-conductance calcium-activated potassium channel KCa3.1 and chloride channel modulate chemokine ligand (CCL19/CCL21)-induced migration of dendritic cells.Translational research : the journal of laboratory and clinical medicine · 2015Article
- Novel CLC3 transcript variants in blood eosinophils and increased CLC3 expression in nasal lavage and blood eosinophils of asthmatics.Immunity, inflammation and disease · 2014Article
- Hydrodynamic cellular volume changes enable glioma cell invasion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Lysophosphatidylcholine- and MCP-1-induced chemotaxis of monocytes requires potassium channel activity.Pflugers Archiv : European journal of physiology · 2009Article
- ClC-3 and IClswell are required for normal neutrophil chemotaxis and shape change.The Journal of biological chemistry · 2008Article
- Drosophila bestrophin-1 chloride current is dually regulated by calcium and cell volume.The Journal of general physiology · 2007Article
- Cells move when ions and water flow.Pflugers Archiv : European journal of physiology · 2007Review
- Swelling activated Cl- channels in microglia: Biophysics, pharmacology and role in glutamate release.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Increased adhesion and diapedesis of monocytes appear to be primary initiating factors in the pathophysiology of occlusive vascular diseases, including atherosclerosis and restenosis. However, the underlying mechanisms of transendothelial migration and invasion of monocytes into the blood vessels are not known. Alterations in ion channels on the cell membrane are generally involved in induced changes in shape and volume. In the present study, we investigated the expression and functional role of chloride channels in freshly isolated human blood monocytes. The Cl- currents in whole-cells were measured by the patch-clamp technique. We observed whole cell Cl- currents, which were time-independent and outwardly rectifying. The chloride channel blockers 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and R(+)-[(6,7-dichloro-2-cyclopentyl-2,3-dihydro-2-methyl-1-oxo-1H-inden-5yl)-oxy]acetic acid 94 (IAA94) attenuated the Cl- currents. NPPB and IAA94 also inhibited chemotaxis of monocytes, as measured in Boyden chemotactic chambers, with the same sensitivity. NPPB but not IAA94, increased the cell volume as measured by shape change, and decreased tumour necrosis factor (TNF)-alpha-induced monocyte adhesion to endothelial cells. These results suggest that monocytes contain Cl- channels which regulate transendothelial migration of monocytes, due presumably to an alteration in cell volume.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.