ArticleMolecular and cellular biology2004
Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 91 citations in OpenAlex.
- Deficiency in homologous recombination is associated with changes in cell cycling and morphology in Saccharomyces cerevisiae.Experimental cell research · 2023Article
- Transcription and FACT facilitate the restoration of replication-coupled chromatin assembly defects.Scientific reports · 2023Article
- PIF7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis.The EMBO journal · 2023Article
- Molecular basis of Tousled-Like Kinase 2 activation.Nature communications · 2018Article
- Review
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- Histone H2B mono-ubiquitylation maintains genomic integrity at stalled replication forks.Nucleic acids research · 2016Article
- The Cac1 subunit of histone chaperone CAF-1 organizes CAF-1-H3/H4 architecture and tetramerizes histones.eLife · 2016Article
- Functions of Ubiquitin and SUMO in DNA Replication and Replication Stress.Frontiers in genetics · 2016Review
- Histone modifications as regulators of life and death inMicrobial cell (Graz, Austria) · 2015Review
- Quantitative analysis of triple-mutant genetic interactions.Nature protocols · 2014Article
- The Ddc1-Mec3-Rad17 sliding clamp regulates histone-histone chaperone interactions and DNA replication-coupled nucleosome assembly in budding yeast.The Journal of biological chemistry · 2014Article
- Genome-wide mapping of yeast histone chaperone anti-silencing function 1 reveals its role in condensin binding with chromatin.PloS one · 2014Article
- Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation.Cell reports · 2013Article
- ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis.Plant physiology · 2013Article
- Mutations in non-acid patch residues disrupt H2A.Z's association with chromatin through multiple mechanisms.PloS one · 2013Article
- Cell-cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei.Nucleic acids research · 2012Article
- Histone dosage regulates DNA damage sensitivity in a checkpoint-independent manner by the homologous recombination pathway.Nucleic acids research · 2012Article
- Nucleosome assembly and genome integrity: The fork is the link.Bioarchitecture · 2012Article
- Histone chaperone Asf1 plays an essential role in maintaining genomic stability in fission yeast.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The packaging of the eukaryotic genome into chromatin is likely to be important for the maintenance of genomic integrity. Chromatin structures are assembled onto newly synthesized DNA by the action of chromatin assembly factors, including anti-silencing function 1 (ASF1). To investigate the role of chromatin structure in the maintenance of genomic integrity, we examined budding yeast lacking the histone chaperone Asf1p. We found that yeast lacking Asf1p accumulate in metaphase of the cell cycle due to activation of the DNA damage checkpoint. Furthermore, yeast lacking Asf1p are highly sensitive to mutations in DNA polymerase alpha and to DNA replicational stresses. Although yeast lacking Asf1p do complete DNA replication, they have greatly elevated rates of DNA damage occurring during DNA replication, as indicated by spontaneous Ddc2p-green fluorescent protein foci. The presence of elevated levels of spontaneous DNA damage in asf1 mutants is due to increased DNA damage, rather than the failure to repair double-strand DNA breaks, because asf1 mutants are fully functional for double-strand DNA repair. Our data indicate that the altered chromatin structure in asf1 mutants leads to elevated rates of spontaneous recombination, mutation, and DNA damage foci formation arising during DNA replication, which in turn activates cell cycle checkpoints that respond to DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.