ArticleMolecular and cellular biology2004
Multiple, distant Gata2 enhancers specify temporally and tissue-specific patterning in the developing urogenital system.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Meta-analysis of genome-wide association studies identifies six new Loci for serum calcium concentrations.PLoS genetics · 2013Pooled it
- GATA Transcription Factors: A Cross-Road for Erythropoiesis, Neurodevelopment, and Synucleinopathies.Developmental neurobiology · 2025Review
- Integrative analysis of the ST6GALNAC family identifies GATA2-upregulated ST6GALNAC5 as an adverse prognostic biomarker promoting prostate cancer cell invasion.Cancer cell international · 2023Article
- Noncoding variants alter GATA2 expression in rhombomere 4 motor neurons and cause dominant hereditary congenital facial paresis.Nature genetics · 2023Article
- Article
- Development and Carcinogenesis: Roles of GATA Factors in the Sympathoadrenal and Urogenital Systems.Biomedicines · 2021Review
- A Novel GATA2 Protein Reporter Mouse Reveals Hematopoietic Progenitor Cell Types.Stem cell reports · 2020Article
- EVI1 and GATA2 misexpression induced by inv(3)(q21q26) contribute to megakaryocyte-lineage skewing and leukemogenesis.Blood advances · 2020Article
- Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6GScientific reports · 2019Article
- Identification of Novel Gata3 Distal Enhancers Active in Mouse Embryonic Lens.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- AMolecular and cellular biology · 2018Article
- Reducing Inflammatory Cytokine Production from Renal Collecting Duct Cells by Inhibiting GATA2 Ameliorates Acute Kidney Injury.Molecular and cellular biology · 2017Article
- The role of GATA2 in lethal prostate cancer aggressiveness.Nature reviews. Urology · 2017Review
- Gata3 Hypomorphic Mutant Mice Rescued with a Yeast Artificial Chromosome Transgene Suffer a Glomerular Mesangial Cell Defect.Molecular and cellular biology · 2016Article
- Progenitor stage-specific activity of a cis-acting double GATA motif for Gata1 gene expression.Molecular and cellular biology · 2015Article
- GATA2 regulates body water homeostasis through maintaining aquaporin 2 expression in renal collecting ducts.Molecular and cellular biology · 2014Article
- A remote GATA2 hematopoietic enhancer drives leukemogenesis in inv(3)(q21;q26) by activating EVI1 expression.Cancer cell · 2014Article
- Conditional Gata2 inactivation results in HSC loss and lymphatic mispatterning.The Journal of clinical investigation · 2012Article
- UG4 enhancer-driven GATA-2 and bone morphogenetic protein 4 complementation remedies the CAKUT phenotype in Gata2 hypomorphic mutant mice.Molecular and cellular biology · 2012Article
- Conserved and non-conserved enhancers direct tissue specific transcription in ancient germ layer specific developmental control genes.BMC developmental biology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Transcription factor GATA-2 is expressed in a complex temporally and tissue-specific pattern within the developing embryo. Loss-of-function studies in the mouse showed that GATA-2 activity is first required during very early hematopoiesis. We subsequently showed that a 271-kbp yeast artificial chromosome (YAC) transgene could fully complement the loss of Gata2 hematopoietic function but that these YAC-rescued Gata2 null mutant mice die perinatally due to defective urogenital development. The rescuing YAC did not display appropriate urogenital expression of Gata2, implying the existence of a urogenital-specific enhancer(s) lying outside the boundaries of this transgene. Here we outline a coupled general strategy for regulatory sequence discovery, linking bioinformatics to functional genomics based on the bacterial artificial chromosome (BAC) libraries used to generate the mouse genome sequence. Exploiting this strategy, we screened >1 Mbp of genomic DNA surrounding Gata2 for urogenital enhancer activity. We found that the spatially and tissue-specific functions for Gata2 in the developing urogenital system are conferred by at least three separate regionally and temporally specific urogenital enhancer elements, two of which reside far 3' to the Gata2 structural gene. Including the additional enhancers that were discovered using this strategy (called BAC trap) extends the functional realm of the Gata2 locus to greater than 1 Mbp.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.