ArticleMolecular and cellular biology2004
In vivo analysis of importin alpha proteins reveals cellular proliferation inhibition and substrate specificity.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 81 citations in OpenAlex.
- Decreased Expression of Karyopherin-α 1 is Related to the Malignant Degree of Cervical Cancer and is Critical for the Proliferation of Hela Cells.Pathology oncology research : POR · 2022Article
- An Arabidopsis nonhost resistance gene,Current research in microbial sciences · 2022Article
- Enterovirus A71 2B Inhibits Interferon-Activated JAK/STAT Signaling by Inducing Caspase-3-Dependent Karyopherin-α1 Degradation.Frontiers in microbiology · 2021Article
- Article
- Importin α1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons.PLoS pathogens · 2018Article
- Chromosomal passenger complex hydrodynamics suggests chaperoning of the inactive state by nucleoplasmin/nucleophosmin.Molecular biology of the cell · 2017Article
- Article
- Identification of the nuclear localisation signal of O-GlcNAc transferase and its nuclear import regulation.Scientific reports · 2016Article
- Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase.Molecular biology of the cell · 2016Article
- Nuclear import of the thyroid hormone receptor α1 is mediated by importin 7, importin β1, and adaptor importin α1.Molecular and cellular endocrinology · 2016Article
- Article
- Identification of importin α 7 specific transport cargoes using a proteomic screening approach.Molecular & cellular proteomics : MCP · 2014Article
- Nuclear import of aristaless-related homeobox protein via its NLS1 regulates its transcriptional function.Molecular and cellular biochemistry · 2013Article
- Serotype-specific differences in dengue virus non-structural protein 5 nuclear localization.The Journal of biological chemistry · 2013Article
- The functional role of the novel biomarker karyopherin α 2 (KPNA2) in cancer.Cancer letters · 2013Review
- Identification of potential biomarkers from microarray experiments using multiple criteria optimization.Cancer medicine · 2013Article
- Genealogy of an ancient protein family: the Sirtuins, a family of disordered members.BMC evolutionary biology · 2013Article
- Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint.Journal of proteome research · 2013Article
- Article
- Hop-on hop-off: importin-α-guided tours to the nucleus in innate immune signaling.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The "classical" nuclear import pathway depends on importins alpha and beta. Humans have only one importin beta, while six alpha importins have been described. Whether or not distinct alpha importins are essential for specific import pathways in living human cells is unclear. We used RNA interference technology to specifically down-regulate the expression of ubiquitously expressed human alpha importins in HeLa cells. Down-regulation of importins alpha3, alpha5, alpha7, and beta strongly inhibited HeLa cell proliferation, while down-regulation of importins alpha1 and alpha4 had only a minor effect or no effect. Nucleoplasmin import was not prevented by down-regulation of any alpha importin, indicating that the importin alpha/beta pathway was generally not affected. In contrast, importin alpha3 or alpha5 down-regulation specifically inhibited the nuclear import of the Ran guanine nucleotide exchange factor, RCC1. Coinjection of recombinant alpha importins and RCC1 into down-regulated cells demonstrated that these transport defects were specifically caused by the limited availability of importin alpha3 in both cases. Thus, importin alpha3 is the only alpha importin responsible for the classical nuclear import of RCC1 in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.