ArticleJournal of virology2004
Expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein GN.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Structural and Functional Analysis of Groundnut bud necrosis virus (GBNV) Using Computational and Biochemical Approaches.Molecular biotechnology · 2025Article
- Protein landscape of the brush border membrane of first instar larvae of Frankliniella occidentalis, the western flower thrips.PloS one · 2025Article
- Voltage-dependent anion channel 2 (VDAC2) facilitates the accumulation of rice stripe virus in the vector Laodelphax striatellus.Virus research · 2023Article
- Polerovirus N-terminal readthrough domain structures reveal molecular strategies for mitigating virus transmission by aphids.Nature communications · 2022Article
- HMG-Like DSP1 Mediates Immune Responses of the Western Flower Thrips (Frontiers in immunology · 2022Article
- Article
- The Bunyavirales: The Plant-Infecting Counterparts.Viruses · 2021Review
- Recent Advances in Bunyavirus Glycoprotein Research: Precursor Processing, Receptor Binding and Structure.Viruses · 2021Review
- Crystal structure of tomato spotted wilt virus GProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A global invasion by the thrip, Frankliniella occidentalis: Current virus vector status and its management.Insect science · 2020Review
- Thrips as the Transmission Bottleneck for Mixed Infection of TwoPlants (Basel, Switzerland) · 2020Article
- Changes in the GVirus genes · 2020Article
- Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus.Journal of virology · 2019Article
- In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector,Virusdisease · 2019Article
- Tenuivirus utilizes its glycoprotein as a helper component to overcome insect midgut barriers for its circulative and propagative transmission.PLoS pathogens · 2019Article
- Vector Competence of Thrips Species to Transmit Soybean Vein Necrosis Virus.Frontiers in microbiology · 2019Article
- Plant Virus-Insect Vector Interactions: Current and Potential Future Research Directions.Viruses · 2016Review
- Cell Walls and the Convergent Evolution of the Viral Envelope.Microbiology and molecular biology reviews : MMBR · 2015Review
- Complete Genome Sequence of Mulberry Vein Banding Associated Virus, a New Tospovirus Infecting Mulberry.PloS one · 2015Article
- Plant pest destruction goes viral.Nature biotechnology · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tomato spotted wilt virus (TSWV), a member of the Tospovirus genus within the Bunyaviridae, is an economically important plant pathogen with a worldwide distribution. TSWV is transmitted to plants via thrips (Thysanoptera: Thripidae), which transmit the virus in a persistent propagative manner. The envelope glycoproteins, G(N) and G(C), are critical for the infection of thrips, but they are not required for the initial infection of plants. Thus, it is assumed that the envelope glycoproteins play important roles in the entry of TSWV into the insect midgut, the first site of infection. To directly test the hypothesis that G(N) plays a role in TSWV acquisition by thrips, we expressed and purified a soluble, recombinant form of the G(N) protein (G(N)-S). The expression of G(N)-S allowed us to examine the function of G(N) in the absence of other viral proteins. We detected specific binding to thrips midguts when purified G(N)-S was fed to thrips in an in vivo binding assay. The TSWV nucleocapsid protein and human cytomegalovirus glycoprotein B did not bind to thrips midguts, indicating that the G(N)-S-thrips midgut interaction is specific. TSWV acquisition inhibition assays revealed that thrips that were concomitantly fed purified TSWV and G(N)-S had reduced amounts of virus in their midguts compared to thrips that were fed TSWV only. Our findings that G(N)-S binds to larval thrips guts and decreases TSWV acquisition provide evidence that G(N) may serve as a viral ligand that mediates the attachment of TSWV to receptors displayed on the epithelial cells of the thrips midgut.
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