ArticleJournal of virology2004
Human cytomegalovirus 5-kilobase immediate-early RNA is a stable intron.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.Viruses · 2025Article
- Shaping the host cell environment with viral noncoding RNAs.Seminars in cell & developmental biology · 2023Review
- Circular stable intronic RNAs possess distinct biological features and are deregulated in bladder cancer.NAR cancer · 2023Article
- Review
- Functional and molecular dissection of HCMV long non-coding RNAs.Scientific reports · 2022Article
- Epigenetic reprogramming of host and viral genes by Human Cytomegalovirus infection in Kasumi-3 myeloid progenitor cells at early times post-infection.Journal of virology · 2021Article
- Article
- Idiosyncrasies of Viral Noncoding RNAs Provide Insights into Host Cell Biology.Annual review of virology · 2019Review
- Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus.Virology · 2019Article
- Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons.Journal of neurovirology · 2018Article
- ShapeShifter: a novel approach for identifying and quantifying stable lariat intronic species in RNAseq data.Quantitative biology (Beijing, China) · 2018Article
- Long Non-Coding RNAs: Novel Players in Regulation of Immune Response Upon Herpesvirus Infection.Frontiers in immunology · 2018Review
- Roles of LncRNAs in Viral Infections.Frontiers in cellular and infection microbiology · 2017Review
- Stable intronic sequence RNAs (sisRNAs): a new layer of gene regulation.Cellular and molecular life sciences : CMLS · 2016Review
- Functional Dissection of an Alternatively Spliced Herpesvirus Gene by Splice Site Mutagenesis.Journal of virology · 2016Article
- Long noncoding RNAs in innate immunity.Cellular & molecular immunology · 2016Review
- Viral noncoding RNAs: more surprises.Genes & development · 2015Review
- EBV Noncoding RNAs.Current topics in microbiology and immunology · 2015Review
- Identification of the alternative splicing of the UL49 locus of human cytomegalovirus.BioMed research international · 2015Article
- Stability determinants of murine cytomegalovirus long noncoding RNA7.2.Journal of virology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Immediate-early viral gene products of human cytomegalovirus (HCMV) are derived from several genomic loci and largely serve to establish a cellular environment conducive to viral replication. We have further examined an unusual immediate-early transcript known as the 5-kb RNA, concluding that it is a stable intron encoded by HCMV. The 5-kb RNA is highly AT rich in sequence and lacks open reading frames likely to be translated into protein. We confirmed the absence of polyadenylation of the transcript and showed that it is primarily nuclear localized during viral infection. We mapped the 5' end of the 5-kb RNA to a consensus splice donor site and localized the 3' end in the vicinity of a splice acceptor site. In transfection studies, we showed that the 5-kb RNA can be spliced from a heterologous primary transcript. Using bacterial artificial chromosome technology, we constructed a viral recombinant containing a mutation in the 5' splice donor site that defines the 5' end of the RNA and found that this mutation eliminates expression of the 5-kb RNA during viral infection. This mutant grows in human fibroblasts without complementation. Taken together, these data support the conclusion that the 5-kb RNA is a stable intron expressed by HCMV.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.