ArticlePlant physiology2004
Ca2+-permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilaments.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 135 citations in OpenAlex.
- Synthetic ion channel inhibitors enhance plant drought tolerance.Nature communications · 2026Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Genome-Wide Analysis of Cyclic Nucleotide-Gated Channel Genes Related to Pollen Development in Rice.Plants (Basel, Switzerland) · 2022Article
- Research on the Molecular Interaction Mechanism between Plants and Pathogenic Fungi.International journal of molecular sciences · 2022Review
- Towards Understanding the Involvement of HMolecules (Basel, Switzerland) · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- P2K1 Receptor, Heterotrimeric Gα Protein and CNGC2/4 Are Involved in Extracellular ATP-Promoted Ion Influx in the Pollen ofPlants (Basel, Switzerland) · 2021Article
- Dynamics of Pollen Activation and the Role of HPlants (Basel, Switzerland) · 2020Article
- Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg.Plants (Basel, Switzerland) · 2019Review
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
- Extracellular ATP promoted pollen germination and tube growth of Nicotiana tabacum through promoting KPlant reproduction · 2018Article
- Calcium dynamics in tomato pollen tubes using the Yellow Cameleon 3.6 sensor.Plant reproduction · 2018Article
- ROS-Activated Ion Channels in Plants: Biophysical Characteristics, Physiological Functions and Molecular Nature.International journal of molecular sciences · 2018Review
- Simultaneous imaging and functional studies reveal a tight correlation between calcium and actin networks.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation.BMC plant biology · 2017Article
- Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness.The New phytologist · 2017Article
- Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes.Plant physiology · 2017Review
- Cyclic nucleotide-gated channel 18 is an essential Ca2+ channel in pollen tube tips for pollen tube guidance to ovules in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach.Planta · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic free Ca2+ and actin microfilaments play crucial roles in regulation of pollen germination and tube growth. The focus of this study is to test the hypothesis that Ca2+ channels, as well as channel-mediated Ca2+ influxes across the plasma membrane (PM) of pollen and pollen tubes, are regulated by actin microfilaments and that cytoplasmic Ca2+ in pollen and pollen tubes is consequently regulated. In vitro Arabidopsis (Arabidopsis thaliana) pollen germination and tube growth were significantly inhibited by Ca2+ channel blockers La3+ or Gd3+ and F-actin depolymerization regents. The inhibitory effect of cytochalasin D (CD) or cytochalasin B (CB) on pollen germination and tube growth was enhanced by increasing external Ca2+. Ca2+ fluorescence imaging showed that addition of actin depolymerization reagents significantly increased cytoplasmic Ca2+ levels in pollen protoplasts and pollen tubes, and that cytoplasmic Ca2+ increase induced by CD or CB was abolished by addition of Ca2+ channel blockers. By using patch-clamp techniques, we identified the hyperpolarization-activated inward Ca2+ currents across the PM of Arabidopsis pollen protoplasts. The activity of Ca2+-permeable channels was stimulated by CB or CD, but not by phalloidin. However, preincubation of the pollen protoplasts with phalloidin abolished the effects of CD or CB on the channel activity. The presented results demonstrate that the Ca2+-permeable channels exist in Arabidopsis pollen and pollen tube PMs, and that dynamic actin microfilaments regulate Ca2+ channel activity and may consequently regulate cytoplasmic Ca2+.
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