ArticleArchives of biochemistry and biophysics2004
Gene expression profiles in rat intestine identify pathways for 1,25-dihydroxyvitamin D(3) stimulated calcium absorption and clarify its immunomodulatory properties.
Article in Archives of biochemistry and biophysics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 146 citations in OpenAlex.
- Vitamin D and Ocular Diseases: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Effects of Supplemental Calcium and Vitamin D on Circulating Biomarkers of Gut Barrier Function in Patients with Colon Adenoma: A Randomized Clinical Trial.Cancer prevention research (Philadelphia, Pa.) · 2021Trial
- Study on the Calcium Transport-Promoting Property and Mechanism of the Peptide-Calcium Complex DEEENDQVK-Ca Based on a Caco-2 Monolayer Model.Foods (Basel, Switzerland) · 2025Article
- The Molecular Biology of Placental Transport of Calcium to the Human Foetus.International journal of molecular sciences · 2025Review
- Analysis of 1,25-dihydroxyvitamin D genomic action in human enteroids and colonoids reveals multiple regulatory effects of vitamin D in human intestinal physiology.Frontiers in endocrinology · 2025Article
- Absence of claudin-3 does not alter intestinal absorption of phosphate in mice.Pflugers Archiv : European journal of physiology · 2024Article
- Periparturient Mineral Metabolism: Implications to Health and Productivity.Animals : an open access journal from MDPI · 2024Review
- Review
- Vitamin D: A Critical Regulator of Intestinal Physiology.JBMR plus · 2021Review
- Article
- Vitamin D as a Potential Preventive Agent For Young Women's Breast Cancer.Cancer prevention research (Philadelphia, Pa.) · 2021Review
- Modulation of Intestinal Phosphate Transport in Young Goats Fed a Low Phosphorus Diet.International journal of molecular sciences · 2021Article
- Hepcidin induces intestinal calcium uptake while suppressing iron uptake in Caco-2 cells.PloS one · 2021Article
- Analysis of 1,25-Dihydroxyvitamin DMolecular and cellular biology · 2020Article
- The Role of Vitamin D in Small Animal Bone Metabolism.Metabolites · 2020Review
- Intestinal CaWorld journal of gastroenterology · 2020Review
- Vitamin D and the intestine: Review and update.The Journal of steroid biochemistry and molecular biology · 2020Review
- Current evidence for vitamin D in intestinal function and disease.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Influence of the concentration of dietary digestible calcium on growth performance, bone mineralization, plasma calcium, and abundance of genes involved in intestinal absorption of calcium in pigs from 11 to 22 kg fed diets with different concentrations of digestible phosphorus.Journal of animal science and biotechnology · 2019Article
- Lowered dietary phosphorus affects intestinal and renal gene expression to maintain mineral homeostasis with immunomodulatory implications in weaned piglets.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarray technology has been used to discover 1,25-dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)) induced gene expression changes in rat small intestine in vivo. Here, we report gene expression changes related to intestinal absorption or transport, the immune system and angiogenesis in response to 1,25-(OH)(2)D(3). Vitamin D deficient rats were intrajugularly given vehicle or vehicle containing 730 ng of 1,25-(OH)(2)D(3)/kg of body weight. Intestinal mRNA was harvested from duodenal mucosa at 15 min, 1, 3, and 6 h post-injection and studied by Affymetrix microarrays. Genes significantly affected by 1,25-(OH)(2)D(3) were confirmed by quantitative RT-PCR with remarkable agreement. The most strongly affected gene in intestine was CYP24 with 97-fold increase at 6 h post-1,25-(OH)(2)D(3) treatment. Intestinal calcium absorption genes: TRPV5, TRPV6, calbindin D(9k), and Ca(2+) dependent ATPase all were up-regulated in response to 1,25-(OH)(2)D(3), supporting the currently accepted mechanism of 1,25-(OH)(2)D(3) induced transcellular calcium transport. However, a 1,25-(OH)(2)D(3) suppression of several intra-/intercellular matrix modeling proteins such as sodium/potassium ATPase, claudin 3, aquaporin 8, cadherin 17, and RhoA suggests a vitamin D regulation of tight junction permeability and paracellular calcium transport. Several other genes related to the immune system and angiogenesis whose expression was changed in response to 1,25-(OH)(2)D(3) provided evidence for an immunomodulatory and anti-angiogenic role of 1,25-(OH)(2)D(3).
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Registered trials
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