ArticleThe Plant cell2004
The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 235 papers.
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Who cites it
235 citing papers in PubMed, 630 citations in OpenAlex.
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- An active RNA transport mechanism into plant vacuoles.Nucleic acids research · 2026Article
- Network ontology transcript annotation identifies genetic signals underlying sex determination.Scientific reports · 2026Article
- Structural basis for pH-responsive amino acid transport via SLC7A4.Nature communications · 2026Article
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- Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity.Nature plants · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Cloning, Expression and Functional Characterization ofInternational journal of molecular sciences · 2025Article
- Origin, evolution and diversification of plant caleosins.BMC plant biology · 2025Article
- The clues offered by SNAREs on the vacuoles of plants and animals.Frontiers in plant science · 2025Review
- Cellular and physiological functions of SGR family in gravitropic response in higher plants.Journal of advanced research · 2025Review
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- The Arabidopsis receptor-like kinase WAKL4 limits cadmium uptake via phosphorylation and degradation of NRAMP1 transporter.Nature communications · 2024Article
- Advances in Plant GABA Research: Biological Functions, Synthesis Mechanisms and Regulatory Pathways.Plants (Basel, Switzerland) · 2024Review
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Phototropin2 3'UTR overlaps with the AT5G58150 gene encoding an inactive RLK kinase.BMC plant biology · 2024Article
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
175 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuoles play central roles in plant growth, development, and stress responses. To better understand vacuole function and biogenesis we have characterized the vegetative vacuolar proteome from Arabidopsis thaliana. Vacuoles were isolated from protoplasts derived from rosette leaf tissue. Total purified vacuolar proteins were then subjected either to multidimensional liquid chromatography/tandem mass spectrometry or to one-dimensional SDS-PAGE coupled with nano-liquid chromatography/tandem mass spectrometry (nano-LC MS/MS). To ensure maximum coverage of the proteome, a tonoplast-enriched fraction was also analyzed separately by one-dimensional SDS-PAGE followed by nano-LC MS/MS. Cumulatively, 402 proteins were identified. The sensitivity of our analyses is indicated by the high coverage of membrane proteins. Eleven of the twelve known vacuolar-ATPase subunits were identified. Here, we present evidence of four tonoplast-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), representing each of the four groups of SNARE proteins necessary for membrane fusion. In addition, potential cargo of the N- and C-terminal propeptide sorting pathways, association of the vacuole with the cytoskeleton, and the vacuolar localization of 89 proteins of unknown function are identified. A detailed analysis of these proteins and their roles in vacuole function and biogenesis is presented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.