ArticleThe Plant cell2004
FRAGILE FIBER3, an Arabidopsis gene encoding a type II inositol polyphosphate 5-phosphatase, is required for secondary wall synthesis and actin organization in fiber cells.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 123 citations in OpenAlex.
- The rice BRITTLE CULM 4 gene encodes a membrane protein affecting cellulose synthesis in the secondary cell wall.Plant & cell physiology · 2025Article
- MTS1 regulates rice plant architecture by mediating phosphoinositide metabolism.Plant biotechnology journal · 2025Article
- KRN5b regulates maize kernel row number through mediating phosphoinositol signalling.Plant biotechnology journal · 2024Article
- Genetic interaction between TTG2 and AtPLC1 reveals a role for phosphoinositide signaling in a co-regulated suite of Arabidopsis epidermal pathways.Scientific reports · 2024Article
- Syntenic Cell Wall QTLs as Versatile Breeding Tools: Intraspecific Allelic Variability and Predictability of Biomass Quality Loci in Target Plant Species.Plants (Basel, Switzerland) · 2023Article
- Construction of a high-density genetic map and dissection of genetic architecture of six agronomic traits in tobacco (Frontiers in plant science · 2023Article
- TheeLife · 2022Article
- Creativity comes from interactions: modules of protein interactions in plants.The FEBS journal · 2022Review
- TheFrontiers in plant science · 2022Article
- Genome-Wide Analysis of Alternative Splicing and Non-Coding RNAs Reveal Complicated Transcriptional Regulation inInternational journal of molecular sciences · 2021Article
- A putative tomato inositol polyphosphate 5-phosphatase, Le5PT1, is involved in plant growth and abiotic stress responses.3 Biotech · 2020Article
- The Function of Inositol Phosphatases in Plant Tolerance to Abiotic Stress.International journal of molecular sciences · 2019Review
- Expansion and Functional Divergence of Inositol Polyphosphate 5-Phosphatases in Angiosperms.Genes · 2019Article
- Identification of cucumber circular RNAs responsive to salt stress.BMC plant biology · 2019Article
- WD40 Repeat Proteins: Signalling Scaffold with Diverse Functions.The protein journal · 2018Review
- The cell biology of secondary cell wall biosynthesis.Annals of botany · 2018Review
- Interaction network of core ABA signaling components in maize.Plant molecular biology · 2018Article
- Perturbing phosphoinositide homeostasis oppositely affects vascular differentiation inDevelopment (Cambridge, England) · 2017Article
- Molecular cloning of the tomato Hairless gene implicates actin dynamics in trichome-mediated defense and mechanical properties of stem tissue.Journal of experimental botany · 2016Article
- Brevis plant1, a putative inositol polyphosphate 5-phosphatase, is required for internode elongation in maize.Journal of experimental botany · 2016Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Type II inositol polyphosphate 5-phosphatases (5PTases) in yeast and animals have been known to regulate the level of phosphoinositides and thereby influence various cellular activities, such as vesicle trafficking and actin organization. In plants, little is known about the phosphatases involved in hydrolysis of phosphoinositides, and roles of type II 5PTases in plant cellular functions have not yet been characterized. In this study, we demonstrate that the FRAGILE FIBER3 (FRA3) gene of Arabidopsis thaliana, which encodes a type II 5PTase, plays an essential role in the secondary wall synthesis in fiber cells and xylem vessels. The fra3 mutations caused a dramatic reduction in secondary wall thickness and a concomitant decrease in stem strength. These phenotypes were associated with an alteration in actin organization in fiber cells. Consistent with the defective fiber and vessel phenotypes, the FRA3 gene was found to be highly expressed in fiber cells and vascular tissues in stems. The FRA3 protein is composed of two domains, an N-terminal localized WD-repeat domain and a C-terminal localized 5PTase catalytic domain. In vitro activity assay demonstrated that recombinant FRA3 exhibited phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3, and Ins(1,4,5)P3, with the highest substrate affinity toward PtdIns(4,5)P2. The fra3 missense mutation, which caused an amino acid substitution in the conserved motif II of the 5PTase catalytic domain, completely abolished the FRA3 phosphatase activity. Moreover, the endogenous levels of PtdIns(4,5)2 and Ins(1,4,5)P3 were found to be elevated in fra3 stems. Together, our findings suggest that the FRA3 type II 5PTase is involved in phosphoinositide metabolism and influences secondary wall synthesis and actin organization.
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