ArticleThe Journal of biological chemistry2005
cAMP has distinct acute and chronic effects on aquaporin-5 in lung epithelial cells.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 95 citations in OpenAlex.
- Aquaporin 5 polymorphisms and rate of lung function decline in chronic obstructive pulmonary disease.PloS one · 2010Trial
- Research Progress on the Mechanisms of High Mucus Secretion in the Airway: A Scoping Review.International journal of chronic obstructive pulmonary disease · 2026Article
- AQP5 trafficking is regulated by its C-terminal tail and interaction with prolactin-inducible protein.Biology direct · 2025Article
- Cooperation of aquaporin 5 and the adrenergic system in the initiation of birth in rat model.Heliyon · 2024Article
- Aquaporins in the Cornea.International journal of molecular sciences · 2024Review
- Aquaporin-5 Dynamic Regulation.International journal of molecular sciences · 2023Review
- Protein Structure and Modification of Aquaporins.Advances in experimental medicine and biology · 2023Article
- Molecular mechanisms governing aquaporin relocalisation.Biochimica et biophysica acta. Biomembranes · 2022Review
- Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway.FEBS open bio · 2021Article
- Expression, Distribution and Role of Aquaporins in Various Rhinologic Conditions.International journal of molecular sciences · 2020Review
- Effects of PACAP on Dry Eye Symptoms, and Possible Use for Therapeutic Application.Journal of molecular neuroscience : MN · 2019Review
- Review
- Altered expression of aquaporin 1 and aquaporin 5 in the cornea after primary blast exposure.Molecular vision · 2019Article
- Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress.PloS one · 2017Article
- Rat Aquaporin-5 Is pH-Gated Induced by Phosphorylation and Is Implicated in Oxidative Stress.International journal of molecular sciences · 2016Article
- Aquaporin-5: from structure to function and dysfunction in cancer.Cellular and molecular life sciences : CMLS · 2016Review
- Physiological role of aquaporin 5 in salivary glands.Pflugers Archiv : European journal of physiology · 2016Review
- Effect of dobutamine on lung aquaporin 5 in endotoxine shock-induced acute lung injury rabbit.Journal of thoracic disease · 2015Article
- Article
- Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Aquaporin-5 (AQP5) is present on the apical membrane of epithelial cells in various secretory glands as well as on the apical membrane of the airway epithelium, airway submucosal glands, and type 1 pneumocytes, where it can participate in respiratory tract water homeostasis. We examined the effects of cAMP on AQP5 distribution and abundance. When AQP5-expressing mouse lung epithelial cells were treated with cAMP or the beta-adrenergic agonist terbutaline, a biphasic AQP5 response was observed. Short term (minutes) exposure to cAMP produced internalization of AQP5 off of the membrane and a decrease in protein abundance. Both of these responses were blocked by inhibition of protein kinase A and the decrease in abundance was blocked by chloroquine, indicating lysosome-mediated degradation. Sustained cAMP exposure (hours) produced an increase in membrane localization and increased abundance; these effects were also blocked by protein kinase A inhibition. The beta-adrenergic agonist terbutaline produced changes in AQP5 abundance in mouse trachea and lung, consistent with our findings in cultured epithelial cells. Purified AQP5 protein was phosphorylated by protein kinase A but not protein kinase C or casein kinase II, and aquaporin-5 was phosphorylated in cultured cells after long term (but not short term) exposure to cAMP. These studies indicate that cAMP and beta-adrenergic agonists produce distinct short and long term effects on AQP5 distribution and abundance that may contribute to regulation of lung water homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.