ArticleGenome biology2004
Assembly and characterization of heterochromatin and euchromatin on human artificial chromosomes.
Article in Genome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Genetic and epigenetic regulation of centromeres: a look at HAC formation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Article
- Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.BMC genomics · 2013Article
- Comparative study of artificial chromosome centromeres in human and murine cells.European journal of human genetics : EJHG · 2013Article
- A new generation of human artificial chromosomes for functional genomics and gene therapy.Cellular and molecular life sciences : CMLS · 2013Review
- Functional epialleles at an endogenous human centromere.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cancer-associated alteration of pericentromeric heterochromatin may contribute to chromosome instability.Oncogene · 2012Article
- Assembling pieces of the centromere epigenetics puzzle.Epigenetics · 2012Review
- Rapid de novo centromere formation occurs independently of heterochromatin protein 1 in C. elegans embryos.Current biology : CB · 2011Article
- Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome.Cell host & microbe · 2010Article
- HAC stability in murine cells is influenced by nuclear localization and chromatin organization.BMC cell biology · 2009Article
- Rice as a model for centromere and heterochromatin research.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
- Human centromeric chromatin is a dynamic chromosomal domain that can spread over noncentromeric DNA.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Replication of centromeric heterochromatin in mouse fibroblasts takes place in early, middle, and late S phase.Histochemistry and cell biology · 2006Article
- The evolutionary dynamics of alpha-satellite.Genome research · 2006Article
- Artificial and engineered chromosomes: developments and prospects for gene therapy.Chromosoma · 2005Review
- Engineered human dicentric chromosomes show centromere plasticity.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman centromere regions are characterized by the presence of alpha-satellite DNA, replication late in S phase and a heterochromatic appearance. Recent models propose that the centromere is organized into conserved chromatin domains in which chromatin containing CenH3 (centromere-specific H3 variant) at the functional centromere (kinetochore) forms within regions of heterochromatin. To address these models, we assayed formation of heterochromatin and euchromatin on de novo human artificial chromosomes containing alpha-satellite DNA. We also examined the relationship between chromatin composition and replication timing of artificial chromosomes.
resultsHeterochromatin factors (histone H3 lysine 9 methylation and HP1alpha) were enriched on artificial chromosomes estimated to be larger than 3 Mb in size but depleted on those smaller than 3 Mb. All artificial chromosomes assembled markers of euchromatin (histone H3 lysine 4 methylation), which may partly reflect marker-gene expression. Replication timing studies revealed that the replication timing of artificial chromosomes was heterogeneous. Heterochromatin-depleted artificial chromosomes replicated in early S phase whereas heterochromatin-enriched artificial chromosomes replicated in mid to late S phase.
conclusionsCentromere regions on human artificial chromosomes and host chromosomes have similar amounts of CenH3 but exhibit highly varying degrees of heterochromatin, suggesting that only a small amount of heterochromatin may be required for centromere function. The formation of euchromatin on all artificial chromosomes demonstrates that they can provide a chromosome context suitable for gene expression. The earlier replication of the heterochromatin-depleted artificial chromosomes suggests that replication late in S phase is not a requirement for centromere function.
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