ArticleGenome biology2004
Transcriptome analysis of haploid male gametophyte development in Arabidopsis.
Article in Genome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 373 papers.
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Who cites it
373 citing papers in PubMed, 712 citations in OpenAlex.
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- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.The EMBO journal · 2026Article
- Molecular cloning and the expression profile of two calnexin genes - CNX1 and CNX2 - during pollen development and pollen tube growth in Petunia.BMC plant biology · 2025Article
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- Kinesins control male germ unit assembly for sperm delivery in Arabidopsis.Nature plants · 2025Article
- Dynamic changes in chromatin structure and transcriptional activity in the generative cells of Lilium longiflorum.Journal of plant research · 2025Article
- Scalable eQTL mapping using single-nucleus RNA-sequencing of recombined gametes from a small number of individuals.PLoS biology · 2025Article
- GOLEM: A tool for visualizing the distribution of Gene regulatOry eLEMents within the plant promoters with a focus on male gametophyte.The Plant journal : for cell and molecular biology · 2025Article
- Insights into genetics of floral development in Amborella trichopoda Baill. through genome-wide survey and expression analysis of MADS-Box transcription factors.Scientific reports · 2025Article
- Global identification and regulatory network analysis reveal the significant roles of lncRNAs during anther and pollen development in Arabidopsis.Plant cell reports · 2025Article
- Fine mapping and expression characteristics analysis of male-sterile gene BrRNR1 in Chinese cabbage (Brassica rapa L. ssp. pekinensis).BMC plant biology · 2025Article
- Proline and ROS: A Unified Mechanism in Plant Development and Stress Response?Plants (Basel, Switzerland) · 2024Review
- Selfing Shapes Fixation of a Mutant Allele Under Flux Equilibrium.Genome biology and evolution · 2024Article
- Arabidopsis KNS3 and its two homologs mediate endoplasmic reticulum-to-plasma membrane traffic of boric acid channels.Journal of experimental botany · 2024Article
- The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis.Plant reproduction · 2024Article
- Genome-wide investigation of the LARP gene family: focus on functional identification and transcriptome profiling of ZmLARP6c1 in maize pollen.BMC plant biology · 2024Article
313 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe haploid male gametophyte generation of flowering plants consists of two- or three-celled pollen grains. This functional specialization is thought to be a key factor in the evolutionary success of flowering plants. Moreover, pollen ontogeny is also an attractive model in which to dissect cellular networks that control cell growth, asymmetric cell division and cellular differentiation. Our objective, and an essential step towards the detailed understanding of these processes, was to comprehensively define the male haploid transcriptome throughout development.
resultsWe have developed staged spore isolation procedures for Arabidopsis and used Affymetrix ATH1 genome arrays to identify a total of 13,977 male gametophyte-expressed mRNAs, 9.7% of which were male-gametophyte-specific. The transition from bicellular to tricellular pollen was accompanied by a decline in the number of diverse mRNA species and an increase in the proportion of male gametophyte-specific transcripts. Expression profiles of regulatory proteins and distinct clusters of coexpressed genes were identified that could correspond to components of gametophytic regulatory networks. Moreover, integration of transcriptome and experimental data revealed the early synthesis of translation factors and their requirement to support pollen tube growth.
conclusionsThe progression from proliferating microspores to terminally differentiated pollen is characterized by large-scale repression of early program genes and the activation of a unique late gene-expression program in maturing pollen. These data provide a quantum increase in knowledge concerning gametophytic transcription and lay the foundations for new genomic-led studies of the regulatory networks and cellular functions that operate to specify male gametophyte development.
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