ArticleThe Journal of experimental medicine2004
Galpha13 mediates a signal that is essential for proliferation and survival of thymocyte progenitors.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04689347 (Phase 1b/2 Trial of 5-fluorouracil, Leucovorin, Irinotecan in Combination With Temozolomide), which is not on this map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Phase 1b/2 Trial of 5-fluorouracil, Leucovorin, Irinotecan in Combination With Temozolomide (FLIRT) and Bevacizumab for the First-line Treatment of Patients With MGMT Silenced, Microsatellite Stable Metastatic Colorectal Cancer.
Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- S1P-S1PR3-RAS promotes the progression of S1PR3Leukemia · 2023Article
- GαFrontiers in cell and developmental biology · 2022Review
- Critical regulation of follicular helper T cell differentiation and function by GαProceedings of the National Academy of Sciences of the United States of America · 2021Article
- GαExperimental & molecular medicine · 2020Review
- Determinant role for the gep oncogenes, Gα12/13, in ovarian cancer cell proliferation and xenograft tumor growth.Genes & cancer · 2015Article
- Implications of non-canonical G-protein signaling for the immune system.Cellular signalling · 2014Review
- Regulation of IL-2 gene expression by Siva and FOXP3 in human T cells.BMC immunology · 2011Article
- Gα13 and Rho mediate endosomal trafficking of CXCR4 into Rab11+ vesicles upon stromal cell-derived factor-1 stimulation.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Selective targeting of leukemic cell growth in vivo and in vitro using a gene silencing approach to diminish S-adenosylmethionine synthesis.The Journal of biological chemistry · 2008Article
- Unique signaling properties of CTAR1 in LMP1-mediated transformation.Journal of virology · 2007Article
- TSLP is involved in expansion of early thymocyte progenitors.BMC immunology · 2007Article
- G protein regulation of MAPK networks.Oncogene · 2007Review
- Citron kinase, a RhoA effector, enhances HIV-1 virion production by modulating exocytosis.Traffic (Copenhagen, Denmark) · 2006Article
- Chemoattractant receptor signaling and the control of lymphocyte migration.Immunologic research · 2006Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
G protein signaling via the Galpha12 family (Galpha12 and Galpha13) has not been well studied in T cells. To investigate whether Galpha12 and Galpha13 are involved in thymopoiesis, we expressed the regulator of G protein signaling domain of p115RhoGEF to inhibit Galpha12 and Galpha13 during thymopoiesis. Fetal thymus organ cultures seeded with p115DeltaDH-expressing progenitor cells showed impaired thymopoiesis with a block at the CD4-CD8-CD44-CD25+ (DN3) stage. Using Galpha13 or Galpha12 minigenes, we demonstrated that Galpha13, but not Galpha12, is required for thymopoiesis. T progenitor cells expressing p115DeltaDH showed reduced proliferation and increased cell death. T cell receptor stimulation of the fetal thymus organ cultures did not rescue the block. Overexpression of the antiapoptotic gene Bcl2 rescued the defect in DN3 cells and partially rescued T cell development. Therefore, Galpha13-mediated signaling is necessary in early thymocyte proliferation and survival.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.