ArticleNucleic acids research2004
Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- The Alteromonas macleodii ribosome enables consecutive incorporation of bulky D-amino acids into peptides.Nucleic acids research · 2026Article
- Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome.Nucleic acids research · 2023Article
- Differential Contribution of Protein Factors and 70S Ribosome to Elongation.International journal of molecular sciences · 2021Article
- Article
- Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system.Scientific reports · 2018Article
- Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Structure of the protein core of translation initiation factor 2 in apo, GTP-bound and GDP-bound forms.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components.Nucleic acids research · 2012Article
- Large facilities and the evolving ribosome, the cellular machine for genetic-code translation.Journal of the Royal Society, Interface · 2009Review
- Structural and functional studies of the Thermus thermophilus 16S rRNA methyltransferase RsmG.RNA (New York, N.Y.) · 2009Article
- Oxidatively modified proteins in Alzheimer's disease (AD), mild cognitive impairment and animal models of AD: role of Abeta in pathogenesis.Acta neuropathologica · 2009Review
- Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine.Journal of molecular biology · 2009Article
- Kinetic and thermodynamic studies of peptidyltransferase in ribosomes from the extreme thermophile Thermus thermophilus.RNA (New York, N.Y.) · 2008Article
- RNA chaperone activity of L1 ribosomal proteins: phylogenetic conservation and splicing inhibition.Nucleic acids research · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Ribosomes from the extreme thermophile Thermus thermophilus are capable of translation in a coupled transcription-translation system derived from Escherichia coli. At 45 degrees C, T.thermophilus ribosomes translate at approximately 25-30% of the maximal rate of E.coli ribosomes, and synthesize full-length protein. T.thermophilus and E.coli subunits can be combined to effect translation, with the spectrum of proteins produced depending upon the source of the 30S subunit. In this system, T.thermophilus ribosomes function in concert with E.coli translational factors and tRNAs, with elongation and release factors being supplied from the E.coli extract, and purified initiation factors (IFs) being added exogenously. Cloned and purified T.thermophilus IF1, IF2 and IF3 supported the synthesis of the same products in vitro as the E.coli factors, although the relative levels of some polypeptides were factor dependent. We conclude that, at least between these two phylogenetically distant species, translational factor function and subunit-subunit interactions are conserved. This functional compatibility is remarkable given the extreme and highly divergent environments to which these species have adapted.
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