ArticleJournal of clinical microbiology2004
Detection of Campylobacter spp. in chicken fecal samples by real-time PCR.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed.
- Environmental Investigation of a Campylobacteriosis Outbreak Among Wedding Attendees - York County, Pennsylvania, 2025.MMWR. Morbidity and mortality weekly report · 2026Article
- Multi-technique characterization of gold nanoparticle-based DNA biosensor with smartphone-enabled photometry for accelerated detection of Campylobacter jejuni.Journal of biological engineering · 2026Article
- Antibiotic resistance profile of Campylobacter species recovered from some freshwater milieu in the Eastern Cape Province, South Africa.BMC microbiology · 2025Article
- Raw Milk as a Source ofPathogens (Basel, Switzerland) · 2025Article
- Bacterial gastroenteritis in the world of culture-independent diagnostic testing: a study to evaluate the kinetics of bacterial shedding by culture and CIDT.Microbiology spectrum · 2025Observational
- Potential shortening of testing time by combination of loop-mediated isothermal amplification and enrichment culture for detection of Salmonella spp. in cattle farms.The Journal of veterinary medical science · 2025Article
- Meta-Transcriptomic Response to Copper Corrosion in Drinking Water Biofilms.Microorganisms · 2025Article
- High oxygen-modified atmosphere packaging induces a viable but non-culturable state of Campylobacter jejuni in chicken meat.The Journal of veterinary medical science · 2025Article
- A prospective analysis of clinical and parasitological outcomes after treatment or a wait-and-see approach of Dientamoeba fragilis infection in an adult general practice population.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Observational
- A Bacteriophage Protein-Based Impedimetric Electrochemical Biosensor for the Detection ofBiosensors · 2024Article
- Foodborne pathogenic bacteria in wild European hedgehogs (Erinaceus europaeus).Acta veterinaria Scandinavica · 2024Article
- Role of Maternal Antibodies in the Protection of Broiler Chicks againstAnimals : an open access journal from MDPI · 2024Article
- Design of dot-blot hybridization assay for simultaneous detection ofAnnals of medicine and surgery (2012) · 2024Article
- Reducing Campylobacter colonization in broilers by active immunization of naive broiler breeders using a bacterin and subunit vaccine.Poultry science · 2023Article
- Quantifying Enteropathogen Contamination along Chicken Value Chains in Maputo, Mozambique: A Multidisciplinary and Mixed-Methods Approach to Identifying High Exposure Settings.Environmental health perspectives · 2023Article
- Monitoring within-farm transmission dynamics of antimicrobial-resistant Campylobacter in dairy cattle using broth microdilution and long-read whole genome sequencing.Scientific reports · 2023Article
- Genomic Characterization and Wetland Occurrence of a NovelMicroorganisms · 2023Article
- Simultaneous Detection ofPathogens (Basel, Switzerland) · 2023Article
- Rapid and sensitive detection of gram-negative bacteria using surface-immobilized polymyxin B.PloS one · 2023Article
- A One Health real-time surveillance system for nowcastingEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2022Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time PCR assay for detecting thermophilic Campylobacter spp. directly in chicken feces has been developed. DNA was isolated from fecal material by using magnetic beads followed by PCR with a prealiquoted PCR mixture, which had been stored at -18 degrees C. Campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 CFU/ml, in a fecal suspension. A bacterial internal control was added before DNA extraction to control both DNA isolation and the presence of PCR inhibitors in the samples. The assay was performed on 111 swab samples from a Danish surveillance program and compared to conventional culturing using selective enrichment. There was no statistically significant difference in performance between real-time PCR and culture by selective enrichment, and the diagnostic specificity was 0.96 with an agreement of 0.92. Therefore, the assay should be useful for screening poultry flocks for the presence of Campylobacter.
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Registered trials
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