ArticleJournal of clinical microbiology2004
Mycobacterium africanum genotyping using novel spacer oligonucleotides in the direct repeat locus.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Comparison of in silico predicted Mycobacterium tuberculosis spoligotypes and lineages from whole genome sequencing data.Scientific reports · 2023Article
- Review of Diagnostic Tests for Detection ofFrontiers in veterinary science · 2021Review
- Unexpected Genomic and Phenotypic Diversity of Mycobacterium africanum Lineage 5 Affects Drug Resistance, Protein Secretion, and Immunogenicity.Genome biology and evolution · 2018Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Article
- A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia.BMC infectious diseases · 2011Article
- Spoligotype profile of Mycobacterium tuberculosis complex strains from HIV-positive and -negative patients in Nigeria: a comparative analysis.Journal of clinical microbiology · 2011Article
- Distinct genotypic profiles of the two major clades of Mycobacterium africanum.BMC infectious diseases · 2010Article
- Unexpectedly high proportion of ancestral Manu genotype Mycobacterium tuberculosis strains cultured from tuberculosis patients in Egypt.Journal of clinical microbiology · 2009Article
- A single-step sequencing method for the identification of Mycobacterium tuberculosis complex species.PLoS neglected tropical diseases · 2008Article
- Novel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex.Journal of bacteriology · 2006Article
- Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology.BMC microbiology · 2006Article
- Discriminatory power and reproducibility of novel DNA typing methods for Mycobacterium tuberculosis complex strains.Journal of clinical microbiology · 2005Article
- Molecular evolutionary history of tubercle bacilli assessed by study of the polymorphic nucleotide within the nitrate reductase (narGHJI) operon promoter.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study involves a first evaluation of 25 novel spacer oligonucleotides in addition to the 43 routine spacers for molecular characterization of a panel of 65 isolates of tubercle bacilli from different geographic origins that were initially classified as Mycobacterium africanum based on phenotypic characters. The 68-spacer format defined four additional patterns, and three groups were identified. The relatively homogeneous groups A1 and A2 included strains from West Africa, and A3-1 included strains from East Africa. The presence of deletion region RD9 confirmed the reclassification of the M. africanum subtype II spoligopattern within group A3-1 as Mycobacterium tuberculosis. These isolates may represent a diverging branch of M. tuberculosis in Africa. The use of new spacers also suggested an undergoing evolution of M. africanum subtype I in West Africa. Our results showed that the strain differentiation within the M. tuberculosis complex is improved by using novel spacers, and extensive studies using new-generation spoligotyping may be helpful to better understand the evolution of M. africanum.
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