Evidence map›Paper›PMID 15528683›Full record

ArticleJournal of clinical microbiology2004

Evaluation of the etest method using Mueller-Hinton agar with glucose and methylene blue for determining amphotericin B MICs for 4,936 clinical isolates of Candida species.

M A Pfaller, L Boyken, S A Messer, S Tendolkar, R J Hollis, D J Diekema

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Relative Abundances of Candida albicans and Candida glabrata inApplied and environmental microbiology · 2018
    Article
  3. Article
  4. Article
  5. Canadian clinical practice guidelines for invasive candidiasis in adults.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2010
    Article
  6. Mutants with heteroresistance to amphotericin B and fluconazole in Candida.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2009
    Article
  7. Article
  8. Article
  9. Review
  10. Slow response of invasive Candida krusei infection to amphotericin B in a clinical time-kill study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2006
    Article
  11. Use of Mueller-Hinton broth and agar in the germ tube test.Revista do Instituto de Medicina Tropical de Sao Paulo
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

M A PfallerDepartment of Pathology, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA. michael-pfaller@uiowa.edu
L Boyken
S A Messer
S Tendolkar
R J Hollis
D J Diekema
University of Iowa · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 microg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of Candida spp. was assessed against that of Etest using RPMI agar with 2% glucose (RPG). MICs were determined by Etest in both media for all 4,936 isolates and were read after incubation for 48 h at 35 degrees C. The Candida isolates included C. albicans (n = 2,728), C. glabrata (n = 722), C. parapsilosis (n = 666), C. tropicalis (n = 528), C. krusei (n = 143), C. lusitaniae (n = 54), C. guilliermondii (n = 39), C. pelliculosa (n = 17), C. kefyr (n = 15), C. rugosa (n = 11), C. dubliniensis (n = 5), C. zeylanoides (n = 4), C. lipolytica (n = 3), and C. famata (n = 1). The Etest results with MH-GMB correlated well with those with RPG. Overall agreement was 92.9%, and agreements for individual species were as follows: C. lusitaniae, 98.1%; C. albicans, 95.1%; C. glabrata, 94.3%; C. krusei, 91.6%; C. parapsilosis, 86.6%; and C. tropicalis, 86.4%. The Etest method using MH-GMB appears to be a useful method for determining amphotericin B susceptibilities of Candida species.

Indexed as

AgarAmphotericin BAntifungal AgentsCandidaCandidiasisCulture MediaGlucoseHumansMethylene BlueMicrobial Sensitivity TestsAgarAmphotericin BAntifungal AgentsCulture MediaGlucoseMethylene Blue

Identifiers

PMID15528683
PMCPMC525169
OpenAlexW1998341191

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.