ArticleJournal of clinical microbiology2004
Development of a PCR method for rapid identification of new Streptococcus mutans serotype k strains.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Diversity of Streptococcus mutans serotypes isolated from dental plaque of Brazilian patients with a history of acute myocardial infarction.Journal of applied oral science : revista FOB · 2026Article
- Raman signatures ofFrontiers in microbiology · 2026Article
- Isolation ofJournal of oral microbiology · 2026Article
- Raman signatures ofFrontiers in microbiology · 2026Article
- Evaluation of Streptococcus mutans strains possessing genes encoding collagen-binding proteins in the Japanese population.BMC oral health · 2025Article
- Serotype and distribution of adhesion genes in Streptococcus mutans isolated from people with Parkinson's disease.Odontology · 2025Article
- Streptococcus mutans serotyping, collagen-binding genes and Candida albicans in dentin carious lesions: a molecular approach.Clinical oral investigations · 2025Article
- Polymerase chain reaction-based identification of various serotypes ofJournal of conservative dentistry and endodontics · 2024Article
- Anti-Adherence and Antimicrobial Activities of Silver Nanoparticles against SerotypesMedicina (Kaunas, Lithuania) · 2022Article
- Cnm ofFrontiers in cellular and infection microbiology · 2022Article
- Aptamer Conjugated Indium Phosphide Quantum Dots with a Zinc Sulphide Shell as Photoluminescent Labels forNanomaterials (Basel, Switzerland) · 2021Article
- Phenotypic and Genotypic Characterization of Streptococcus mutans Strains Isolated from Endodontic Infections.Journal of endodontics · 2020Article
- Specific strains of Streptococcus mutans, a pathogen of dental caries, in the tonsils, are associated with IgA nephropathy.Scientific reports · 2019Article
- Comparison of oral flora before and after triple therapy for Helicobacter pylori eradication in patient with gastric disease.Odontology · 2019Article
- Streptococcus mutans serotypes and collagen-binding proteins Cnm/Cbm in children with caries analysed by PCR.Molecular oral microbiology · 2019Article
- Lesions of endodontic origin: An emerging risk factor for coronary heart diseases.Indian heart journal · 2018Review
- Streptococcus Mutans Adhesin Biotypes that Match and Predict Individual Caries Development.EBioMedicine · 2017Article
- Contribution of the Collagen-Binding Proteins of Streptococcus mutans to Bacterial Colonization of Inflamed Dental Pulp.PloS one · 2016Article
- Genotypic and phenotypic heterogeneity inSpringerPlus · 2016Article
- Assessment of clonality and serotypes of Streptococcus mutans among children by multilocus sequence typing.European journal of oral sciences · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a previous study, we isolated and characterized a new serotype k of Streptococcus mutans from human blood and oral cavities. Analysis of the genes involved in biosynthesis of the serotype-specific polysaccharide of serotype k strains revealed that the serotype k-specific nucleotide alignment was commonly present in the 5' region of the rgpF gene (350 bp from the initial sequence) compared to the reference strains, and then a method for rapid identification of serotype k strains was developed by use of PCR with primers designed on the basis of the sequence of the variable region. PCR assays with primers specific for amplification of serotype k strains showed a negative reaction with serotype c, e, and f strains and a positive reaction with serotype k strains, with the sensitivity for identification of the serotype k strains shown to range from 5 to 50 cells. Next, the frequency of positive reactions for serotype k-specific primers was surveyed with DNA taken from saliva samples from 200 subjects (2 to 18 years of age), and 10 of those showed a positive reaction, which was higher than the frequency in our previous survey with a serological method. In addition, all saliva samples from subjects with serotype k strains in our previous study were shown to be positive with the serotype k-specific primers. These results indicate that this new PCR method is effective for identification of subjects with S. mutans serotype k.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.