ArticleApplied and environmental microbiology2004
Saccharomyces cerevisiae engineered for xylose metabolism exhibits a respiratory response.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 173 citations in OpenAlex.
- Valorization of Miscanthus × giganteus hydrolysates into heme-enriched yeast biomass and extract using xylose-fermenting Saccharomyces cerevisiae.Bioresources and bioprocessing · 2026Article
- Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.Microbial cell factories · 2026Article
- Article
- Modular deregulation of central carbon metabolism for efficient xylose utilization in Saccharomyces cerevisiae.Nature communications · 2025Article
- Glucose receptor deletion and engineering: impact on xylose sensing and utilization in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium.Nature communications · 2024Article
- Nicotinic acid availability impacts redox cofactor metabolism in Saccharomyces cerevisiae during alcoholic fermentation.FEMS yeast research · 2024Article
- PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae.PLoS genetics · 2023Article
- Rational and evolutionary engineering of Saccharomyces cerevisiae for production of dicarboxylic acids from lignocellulosic biomass and exploring genetic mechanisms of the yeast tolerance to the biomass hydrolysate.Biotechnology for biofuels and bioproducts · 2022Article
- Deletion ofFrontiers in microbiology · 2022Article
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Exploring metal ion metabolisms to improve xylose fermentation in Saccharomyces cerevisiae.Microbial biotechnology · 2021Article
- The impact of transcription factors Znf1, Sip4, Adr1, Tup1, and Hap4 on xylose alcoholic fermentation in the engineered yeast Saccharomyces cerevisiae.Antonie van Leeuwenhoek · 2021Article
- Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast.Nature communications · 2021Article
- Yeast-Based Biosynthesis of Natural Products From Xylose.Frontiers in bioengineering and biotechnology · 2021Review
- Rewiring yeast metabolism to synthesize products beyond ethanol.Current opinion in chemical biology · 2020Review
- Corrigendum to "Rewiring yeast metabolism to synthesize products beyond ethanol" [Curr Opin Chem Biol 59 (December 2020) 182-192].Current opinion in chemical biology · 2020Article
- Exploring the xylose paradox in Saccharomyces cerevisiae through in vivo sugar signalomics of targeted deletants.Microbial cell factories · 2019Article
- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol inBiotechnology for biofuels · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Native strains of Saccharomyces cerevisiae do not assimilate xylose. S. cerevisiae engineered for d-xylose utilization through the heterologous expression of genes for aldose reductase (XYL1), xylitol dehydrogenase (XYL2), and d-xylulokinase (XYL3 or XKS1) produce only limited amounts of ethanol in xylose medium. In recombinant S. cerevisiae expressing XYL1, XYL2, and XYL3, mRNA transcript levels for glycolytic, fermentative, and pentose phosphate enzymes did not change significantly on glucose or xylose under aeration or oxygen limitation. However, expression of genes encoding the tricarboxylic acid cycle, respiration enzymes (HXK1, ADH2, COX13, NDI1, and NDE1), and regulatory proteins (HAP4 and MTH1) increased significantly when cells were cultivated on xylose, and the genes for respiration were even more elevated under oxygen limitation. These results suggest that recombinant S. cerevisiae does not recognize xylose as a fermentable carbon source and that respiratory proteins are induced in response to cytosolic redox imbalance; however, lower sugar uptake and growth rates on xylose might also induce transcripts for respiration. A petite respiration-deficient mutant (rho degrees ) of the engineered strain produced more ethanol and accumulated less xylitol from xylose. It formed characteristic colonies on glucose, but it did not grow on xylose. These results are consistent with the higher respiratory activity of recombinant S. cerevisiae when growing on xylose and with its inability to grow on xylose under anaerobic conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.