ArticleApplied and environmental microbiology2004
Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 75 citations in OpenAlex.
- Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases.Frontiers in microbiology · 2025Review
- Article
- Detection of Multiplex NASBA RNA Products Using Colorimetric Split G Quadruplex Probes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.Analytical methods : advancing methods and applications · 2017Article
- Nucleic acid-based biotechnologies for food-borne pathogen detection using routine time-intensive culture-based methods and fast molecular diagnostics.Food science and biotechnology · 2016Review
- Point of Care Technologies for HIV.AIDS research and treatment · 2014Review
- Molecular detection and genotyping of noroviruses.Food and environmental virology · 2012Review
- Competitive electrochemiluminescence wash and no-wash immunoassays for detection of serum antibodies to smooth Brucella strains.Clinical and vaccine immunology : CVI · 2009Article
- Evaluation of the persistence of infectious human noroviruses on food surfaces by using real-time nucleic acid sequence-based amplification.Applied and environmental microbiology · 2008Article
- New tools for the study and direct surveillance of viral pathogens in water.Current opinion in biotechnology · 2008Review
- Procedure for rapid concentration and detection of enteric viruses from berries and vegetables.Applied and environmental microbiology · 2007Article
- Real-time detection of noroviruses in surface water by use of a broadly reactive nucleic acid sequence-based amplification assay.Applied and environmental microbiology · 2006Article
- Rapid detection of norovirus from fecal specimens by real-time reverse transcription-loop-mediated isothermal amplification assay.Journal of clinical microbiology · 2006Article
- Diagnosis of hepatitis a virus infection: a molecular approach.Clinical microbiology reviews · 2006Review
- Current and developing technologies for monitoring agents of bioterrorism and biowarfare.Clinical microbiology reviews · 2005Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enteric viruses are currently recognized as one of the most important causes of food-borne disease. Implication of enteric viruses in food-borne outbreaks can be difficult to confirm due to the inadequacy of the detection methods available. In this study, a nucleic acid sequence-based amplification (NASBA) method was developed in a multiplex format for the specific, simultaneous, and rapid detection of epidemiologically relevant human enteric viruses. Three previously reported primer sets were used in a single reaction for the amplification of RNA target fragments of 474, 371, and 165 nucleotides for the detection of hepatitis A virus and genogroup I and genogroup II noroviruses, respectively. Amplicons were detected by agarose gel electrophoresis and confirmed by electrochemiluminescence and Northern hybridization. Endpoint detection sensitivity for the multiplex NASBA assay was approximately 10(-1) reverse transcription-PCR-detectable units (or PFU, as appropriate) per reaction. When representative ready-to-eat foods (deli sliced turkey and lettuce) were inoculated with various concentrations of each virus and processed for virus detection with the multiplex NASBA method, all three human enteric viruses were simultaneously detected at initial inoculum levels of 10(0) to 10(2) reverse transcription-PCR-detectable units (or PFU)/9 cm2 in both food commodities. The multiplex NASBA system provides rapid and simultaneous detection of clinically relevant food-borne viruses in a single reaction tube and may be a promising alternative to reverse transcription-PCR for the detection of viral contamination of foods.
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