ArticleApplied and environmental microbiology2004
Representative freshwater bacterioplankton isolated from Crater Lake, Oregon.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A guide to the natural history of freshwater lake bacteria.Microbiology and molecular biology reviews : MMBR · 2011Pooled it
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- Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.Microbiology spectrum · 2023Article
- A compendium of bacterial and archaeal single-cell amplified genomes from oxygen deficient marine waters.Scientific data · 2023Article
- Unexpected diversity found within benthic microbial mats at hydrothermal springs in Crater Lake, Oregon.Frontiers in microbiology · 2022Article
- High-throughput cultivation based on dilution-to-extinction with catalase supplementation and a case study of cultivating acI bacteria from Lake Soyang.Journal of microbiology (Seoul, Korea) · 2020Article
- Expanding the Diversity of Bacterioplankton Isolates and Modeling Isolation Efficacy with Large-Scale Dilution-to-Extinction Cultivation.Applied and environmental microbiology · 2020Article
- Diversity and distribution of marine heterotrophic bacteria from a large culture collection.BMC microbiology · 2020Article
- Red Sea SAR11 andApplied and environmental microbiology · 2019Article
- The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures.Scientific reports · 2017Article
- Exploring the CultivableFrontiers in microbiology · 2017Article
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- In Situ Microbial Community Succession on Mild Steel in Estuarine and Marine Environments: Exploring the Role of Iron-Oxidizing Bacteria.Frontiers in microbiology · 2016Article
- Stoichiometric flexibility in diverse aquatic heterotrophic bacteria is coupled to differences in cellular phosphorus quotas.Frontiers in microbiology · 2015Article
- Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics.Nature protocols · 2014Article
- Temporal changes and altitudinal distribution of aerobic anoxygenic phototrophs in mountain lakes.Applied and environmental microbiology · 2013Article
- Cell sorting analysis of geographically separated hypersaline environments.Extremophiles : life under extreme conditions · 2013Article
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- Proposal for a method to estimate nutrient shock effects in bacteria.BMC research notes · 2012Article
- Growing unculturable bacteria.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
High-throughput culturing (HTC) methods that rely on dilution to extinction in very-low-nutrient media were used to obtain bacterial isolates from Crater Lake, Oregon. 16S rRNA sequence determination and phylogenetic reconstruction were used to determine the potential ecological significance of isolated bacteria, both in Crater Lake and globally. Fifty-five Crater Lake isolates yielded 16 different 16S rRNA gene sequences. Thirty of 55 (55%) Crater Lake isolates had 16S rRNA gene sequences with 97% or greater similarity to sequences recovered previously from Crater Lake 16S rRNA gene clone libraries. Furthermore, 36 of 55 (65%) Crater Lake isolates were found to be members of widely distributed freshwater groups. These results confirm that HTC is a significant improvement over traditional isolation techniques that tend to enrich for microorganisms that do not predominate in their environment and rarely correlate with 16S rRNA gene clone library sequences. Although all isolates were obtained under dark, heterotrophic growth conditions, 2 of the 16 different groups showed evidence of photosynthetic capability as assessed by the presence of puf operon sequences, suggesting that photoheterotrophy may be a significant process in this oligotrophic, freshwater habitat.
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