ArticleApplied and environmental microbiology2004
Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
63 citing papers in PubMed, 192 citations in OpenAlex.
- Article
- Section-level genome sequencing and comparative genomics ofStudies in mycology · 2025Article
- Genome diversity, population structure and MALDI-TOF MS profiling of Aspergillus oryzae/flavus strains from fermentation and wild environments.BMC genomics · 2025Article
- Molecular Mechanism of Aflatoxin BJournal of fungi (Basel, Switzerland) · 2025Article
- Pre- and post-harvest aflatoxin contamination and management strategies of Aspergillus spoilage in East African Community maize: review of etiology and climatic susceptibility.Mycotoxin research · 2024Review
- Comparative analysis of the genomes and aflatoxin production patterns of three species within the Aspergillus section Flavi reveals an undescribed chemotype and habitat-specific genetic traits.Communications biology · 2024Article
- Comparative Genome Analysis of Japanese Field-IsolatedJournal of fungi (Basel, Switzerland) · 2024Article
- Plant-Entomopathogenic Fungi Interaction: Recent Progress and Future Prospects on Endophytism-Mediated Growth Promotion and Biocontrol.Plants (Basel, Switzerland) · 2024Review
- Aspergillus flavus pangenome (AflaPan) uncovers novel aflatoxin and secondary metabolite associated gene clusters.BMC plant biology · 2024Article
- First record ofFrontiers in microbiology · 2023Article
- Review
- Formation of B- and M-group aflatoxins and precursors by Aspergillus flavus on maize and its implication for food safety.Mycotoxin research · 2022Article
- Review
- Characterization of NonaflatoxigenicMycobiology · 2022Article
- FunOrder: A robust and semi-automated method for the identification of essential biosynthetic genes through computational molecular co-evolution.PLoS computational biology · 2021Article
- Authentication of Aspergillus parasiticus strains in the genome database of the National Center for Biotechnology Information.BMC research notes · 2021Article
- Article
- Characteristics, Occurrence, Detection and Detoxification of Aflatoxins in Foods and Feeds.Foods (Basel, Switzerland) · 2020Review
- Review
- Aflatoxins: Producing-Molds, Structure, Health Issues and Incidence in Southeast Asian and Sub-Saharan African Countries.International journal of environmental research and public health · 2020Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus flavus isolates produce only aflatoxins B1 and B2, while Aspergillus parasiticus and Aspergillus nomius produce aflatoxins B1, B2, G1, and G2. Sequence comparison of the aflatoxin biosynthesis pathway gene cluster upstream from the polyketide synthase gene, pksA, revealed that A. flavus isolates are missing portions of genes (cypA and norB) predicted to encode, respectively, a cytochrome P450 monooxygenase and an aryl alcohol dehydrogenase. Insertional disruption of cypA in A. parasiticus yielded transformants that lack the ability to produce G aflatoxins but not B aflatoxins. The enzyme encoded by cypA has highest amino acid identity to Gibberella zeae Tri4 (38%), a P450 monooxygenase previously shown to be involved in trichodiene epoxidation. The substrate for CypA may be an intermediate formed by oxidative cleavage of the A ring of O-methylsterigmatocystin by OrdA, the P450 monooxygenase required for formation of aflatoxins B1 and B2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.